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dc.contributor.authorRoman-Canal, Berta
dc.contributor.authorTarragona Foradada, Jordi
dc.contributor.authorMoiola, Cristian P.
dc.contributor.authorGatius Calderó, Sònia
dc.contributor.authorBonnin, Sarah
dc.contributor.authorRuiz Miró, Maria
dc.contributor.authorSierra, José Enrique
dc.contributor.authorRufas, Maria
dc.contributor.authorGonzález, Esperanza
dc.contributor.authorPorcel Pérez, José Manuel
dc.contributor.authorGil-Moreno, Antonio
dc.contributor.authorFalcon-Perez, Juan M.
dc.contributor.authorPonomarenko, Julia
dc.contributor.authorMatias-Guiu, Xavier
dc.contributor.authorColás, Eva
dc.description.abstractBackground: Colorectal cancer (CRC) is the third leading cause of cancer-related mortality worldwide. Current systematic methods for diagnosing have inherent limitations so development of a minimally-invasive diagnosis, based on the identification of sensitive biomarkers in liquid biopsies could therefore facilitate screening among population at risk. Methods: In this study, we aim to develop a novel approach to identify highly sensitive and specific biomarkers by investigating the use of extracellular vesicles (EVs) isolated from the peritoneal lavage as a source of potential miRNA diagnostic biomarkers. We isolated EVs by ultracentrifugation from 25 ascitic fluids and 25 peritoneal lavages from non-cancer and CRC patients, respectively. Analysis of the expression of EV-associated miRNAs was performed using Taqman OpenArray technology through which we could detect 371 miRNAs. Results: 210 miRNAs were significantly dysregulated (adjusted p value < 0.05 and abs(logFC) ≥ 1). The top-10 miRNAs, which had the AUC value higher than 0.95, were miRNA-199b-5p, miRNA-150-5p, miRNA-29c-5p, miRNA-218-5p, miRNA-99a-3p, miRNA-383-5p, miRNA-199a-3p, miRNA-193a-5p, miRNA-10b-5p and miRNA-181c-5p. Conclusions: This finding opens the avenue to the use of EV-associated miRNA of peritoneal lavages as an untapped source of biomarkers for CRC.ca_ES
dc.description.sponsorshipEC hold a postdoctoral fellowship from the Departament de Salut of the Generalitat de Catalunya (SLT002/16/00274). This work was supported by grants: Discovery, validation and implementation of biomarkers for Precision Oncology (ISCIII PIE15/00029), CIBERONC (CB16/12/00231 and CB16/12/00328). Grups consolidats de la Generalitat de Catalunya (2017SGR1368 and 2017SGR1661). Work supported by IRBLleida BIOBANK (B.0000682) and Plataforma biobancos PT17/0015/0027.
dc.publisherBioMed Centralca_ES
dc.relation.isformatofReproducció del document publicat a:
dc.relation.ispartofJournal of Translational Medicine, 2019, vol. 17, núm. 208ca_ES
dc.rightscc-by (c) Roman-Canal et al., 2019ca_ES
dc.subjectColorectal cancerca_ES
dc.subjectAscitic fluidca_ES
dc.titleEV-associated miRNAs from peritoneal lavage as potential diagnostic biomarkers in colorectal cancerca_ES

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cc-by (c) Roman-Canal et al., 2019
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