EV-associated miRNAs from peritoneal lavage as potential diagnostic biomarkers in colorectal cancer

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Data de publicació
2019
Autor/a
Roman-Canal, Berta
Tarragona Foradada, Jordi
Moiola, Cristian P.
Gatius Calderó, Sònia
Bonnin, Sarah
Ruiz Miró, Maria
Sierra, José Enrique
Rufas, Maria
González, Esperanza
Porcel Pérez, José Manuel
Gil-Moreno, Antonio
Falcon-Perez, Juan M.
Ponomarenko, Julia
Matias-Guiu, Xavier
Colás, Eva
Citació recomanada
Roman-Canal, Berta; Tarragona Foradada, Jordi; Moiola, Cristian P.; Gatius Calderó, Sònia; Bonnin, Sarah; Ruiz Miró, Maria; ... Colás, Eva. (2019) . EV-associated miRNAs from peritoneal lavage as potential diagnostic biomarkers in colorectal cancer. Journal of Translational Medicine, 2019, vol. 17, núm. 208. https://doi.org/10.1186/s12967-019-1954-8.
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Resum
Background: Colorectal cancer (CRC) is the third leading cause of cancer-related mortality worldwide. Current systematic methods for diagnosing have inherent limitations so development of a minimally-invasive diagnosis, based on the identification of sensitive biomarkers in liquid biopsies could therefore facilitate screening among population at risk. Methods: In this study, we aim to develop a novel approach to identify highly sensitive and specific biomarkers by investigating the use of extracellular vesicles (EVs) isolated from the peritoneal lavage as a source of potential miRNA diagnostic biomarkers. We isolated EVs by ultracentrifugation from 25 ascitic fluids and 25 peritoneal lavages from non-cancer and CRC patients, respectively. Analysis of the expression of EV-associated miRNAs was performed using Taqman OpenArray technology through which we could detect 371 miRNAs. Results: 210 miRNAs were significantly dysregulated (adjusted p value < 0.05 and abs(logFC) ≥ 1). The top-10 miRNAs, which had the AUC value higher than 0.95, were miRNA-199b-5p, miRNA-150-5p, miRNA-29c-5p, miRNA-218-5p, miRNA-99a-3p, miRNA-383-5p, miRNA-199a-3p, miRNA-193a-5p, miRNA-10b-5p and miRNA-181c-5p. Conclusions: This finding opens the avenue to the use of EV-associated miRNA of peritoneal lavages as an untapped source of biomarkers for CRC.
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http://hdl.handle.net/10459.1/68137
DOI
https://doi.org/10.1186/s12967-019-1954-8
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Journal of Translational Medicine, 2019, vol. 17, núm. 208
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