EV-associated miRNAs from peritoneal lavage as potential diagnostic biomarkers in colorectal cancer

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2019Author
Roman-Canal, Berta
Moiola, Cristian P.
Gatius Calderó, Sònia
Bonnin, Sarah
Ruiz Miró, Maria
Sierra, José Enrique
Rufas, Maria
González, Esperanza
Gil-Moreno, Antonio
Falcon-Perez, Juan M.
Ponomarenko, Julia
Colas, Eva
Suggested citation
Roman-Canal, Berta;
Tarragona Foradada, Jordi;
Moiola, Cristian P.;
Gatius Calderó, Sònia;
Bonnin, Sarah;
Ruiz Miró, Maria;
...
Colas, Eva.
(2019)
.
EV-associated miRNAs from peritoneal lavage as potential diagnostic biomarkers in colorectal cancer.
Journal of Translational Medicine, 2019, vol. 17, núm. 208.
https://doi.org/10.1186/s12967-019-1954-8.
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Background: Colorectal cancer (CRC) is the third leading cause of cancer-related mortality worldwide. Current systematic methods for diagnosing have inherent limitations so development of a minimally-invasive diagnosis, based on the identification of sensitive biomarkers in liquid biopsies could therefore facilitate screening among population at risk. Methods: In this study, we aim to develop a novel approach to identify highly sensitive and specific biomarkers by investigating the use of extracellular vesicles (EVs) isolated from the peritoneal lavage as a source of potential miRNA diagnostic biomarkers. We isolated EVs by ultracentrifugation from 25 ascitic fluids and 25 peritoneal lavages from non-cancer and CRC patients, respectively. Analysis of the expression of EV-associated miRNAs was performed using Taqman OpenArray technology through which we could detect 371 miRNAs. Results: 210 miRNAs were significantly dysregulated (adjusted p value < 0.05 and abs(logFC) ≥ 1). The top-10 miRNAs, which had the AUC value higher than 0.95, were miRNA-199b-5p, miRNA-150-5p, miRNA-29c-5p, miRNA-218-5p, miRNA-99a-3p, miRNA-383-5p, miRNA-199a-3p, miRNA-193a-5p, miRNA-10b-5p and miRNA-181c-5p. Conclusions: This finding opens the avenue to the use of EV-associated miRNA of peritoneal lavages as an untapped source of biomarkers for CRC.
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Journal of Translational Medicine, 2019, vol. 17, núm. 208European research projects
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