EV-associated miRNAs from peritoneal lavage as potential diagnostic biomarkers in colorectal cancer
Moiola, Cristian P.
Gatius Calderó, Sònia
Ruiz Miró, Maria
Sierra, José Enrique
Falcon-Perez, Juan M.
MetadataShow full item record
Background: Colorectal cancer (CRC) is the third leading cause of cancer-related mortality worldwide. Current systematic methods for diagnosing have inherent limitations so development of a minimally-invasive diagnosis, based on the identification of sensitive biomarkers in liquid biopsies could therefore
facilitate screening among population at risk. Methods: In this study, we aim to develop a novel approach to identify highly sensitive and specific biomarkers by investigating the use of extracellular vesicles (EVs) isolated from the peritoneal lavage as a source of potential miRNA diagnostic biomarkers. We isolated EVs by ultracentrifugation from 25 ascitic fluids and 25 peritoneal lavages from non-cancer and CRC patients, respectively. Analysis of the expression of EV-associated miRNAs was performed using Taqman OpenArray technology through which we could detect 371 miRNAs. Results: 210 miRNAs were significantly dysregulated (adjusted p value < 0.05 and abs(logFC) ≥ 1). The top-10 miRNAs, which had the AUC value higher than 0.95, were miRNA-199b-5p, miRNA-150-5p, miRNA-29c-5p, miRNA-218-5p, miRNA-99a-3p, miRNA-383-5p, miRNA-199a-3p, miRNA-193a-5p, miRNA-10b-5p and miRNA-181c-5p. Conclusions: This finding opens the avenue to the use of EV-associated miRNA of peritoneal lavages as an untapped source of biomarkers for CRC.
Is part ofJournal of Translational Medicine, 2019, vol. 17, núm. 208
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as cc-by (c) Roman-Canal et al., 2019
Showing items related by title, author, creator and subject.
Roman-Canal, Berta; Moiola, Cristian P.; Gatius Calderó, Sònia; Bonnin, Sarah; Ruiz Miró, Maria; González, Esperanza; González-Tallada, Xavier; Llordella, Ivanna; Hernández, Isabel; Porcel Pérez, José Manuel; Gil-Moreno, Antonio; Falcon-Perez, Juan M.; Ponomarenko, Julia; Matias-Guiu, Xavier; Colas, Eva (MDPI, 2019)Endometrial cancer (EC) is the sixth most common cancer in women worldwide and is responsible for more than 89,000 deaths every year. Mortality is associated with presence of poor prognostic factors at diagnosis, i.e., ...
Roman-Canal, Berta; Moiola, Cristian P.; Gatius Calderó, Sònia; Bonnin, Sarah; Ruiz Miró, Maria; González, Esperanza; Ojanguren, Amaia; Recuero, José Luis; Gil-Moreno, Antonio; Falcón-Pérez, Juan M.; Ponomarenko, Julia; Porcel Pérez, José Manuel; Matias-Guiu, Xavier; Colas, Eva (Nature Research, 2019)Lung cancer is the leading cause of cancer-related deaths among men and women in the world, accounting for the 25% of cancer mortality. Early diagnosis is an unmet clinical issue. In this work, we focused to develop a ...
Dosil Garcia, Maria Alba; Mirantes Barbeito, Cristina; Eritja Sánchez, Núria; Felip, Isidre; Navaridas Fernández de Bobadilla, Raúl; Gatius Calderó, Sònia; Santacana Espasa, Maria; Colàs, Eva; Moiola, Cristian P.; Schoenenberger, Joan Antoni; Encinas Martín, Mario; Garí Marsol, Eloi; Matias-Guiu, Xavier; Dolcet Roca, Xavier (Wiley, 2017-04-28)PTEN is one of the most frequently mutated genes in human cancers. The frequency of PTEN alterations is particularly high in endometrial carcinomas. Loss of PTEN leads to dysregulation of cell division, and promotes the ...