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dc.contributor.authorVila Donat, P.
dc.contributor.authorMarín Sillué, Sònia
dc.contributor.authorSanchís Almenar, Vicente
dc.contributor.authorRamos Girona, Antonio J.
dc.date.accessioned2020-01-20T12:04:18Z
dc.date.available2020-07-21T22:19:14Z
dc.date.issued2019-07-21
dc.identifier.issn0377-8401
dc.identifier.urihttp://hdl.handle.net/10459.1/67827
dc.description.abstractIn the present work different clays have been characterized according to their mycotoxins adsorbent ability. Firstly, 27 bentonite clays from different geographical origins were evaluated, at 0.02% w/v, using an in vitro screening method versus five mycotoxins (aflatoxin B1, AFB1; deoxynivalenol, DON; ochratoxin A, OTA; fumonisin B1, FB1; and, zearalenone, ZEN) by Enzyme- Linked Immunosorbent Assay (ELISA). Subsequently, 7 bentonite clays (6 of which were trioctahedral bentonites) selected from the preliminary test, and 7 commercial adsorbent products were subjected to an in vitro equilibrium adsorption experiment (at 0.02% w/v) against six concentrations of AFB1 (0.02-4 mg/L), and OTA (0.05-1 mg/L) by using simulated gastrointestinal (GI) juices, and successively analysed by HPLC-FD. Equilibrium isotherm functions were fitted to the data by nonlinear regression analysis. In vitro adsorption equilibrium experiments showed that AFB1 adsorption was very high with all the adsorbents tested. In particular, the seven pre-selected bentonites adsorbed most of the AFB1 present at the lower level tested, while only three of these reached more than 50% of OTA adsorption. Adsorption increased inversely to the toxin concentration and both Langmuir and Freundlich isotherm models fitted well to the data. Generally, the pre-selected bentonites (B1-B7) showed better mycotoxin adsorption than commercial products (C1-C7) at all levels of mycotoxins tested. The 10-fold dose increase of the best tri-octahedral bentonite (B4) rendered a more effective adsorption of OTA, reaching almost 75% of adsorption (at pH 5)
dc.description.sponsorshipThe authors are grateful to the following institutions: Spanish government (projects AGL2014-52648-REDT and RTC-2015-3508-2–ERDF. A way to make Europe, funded by the Ministry of Economy and Competitiveness of the Government of Spain and by the European Regional Development Fund of the European Union with the objective of “Promoting technological development, innovation and quality research”) and to XaRTA (The Catalonian Reference Network in Food Technology) for funding. P. Vila-Donat thanks MINECO for her postdoctoral contract.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherElsevier
dc.relationMINECO/PN2013-2016/AGL2014-52648-REDT
dc.relationMINECO/PN2013-2016/RTC-2015-3508-2–ERDF
dc.relation.isformatofVersió postprint del document publicat a: https://doi.org/10.1016/j.anifeedsci.2019.114228
dc.relation.ispartofAnimal Feed Science and Technology, 2019, vol. 255, article number 114228
dc.rightscc-by-nc-nd (c) Elsevier, 2019
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es
dc.subjectmicotoxina
dc.subjectAdsorción
dc.subjectBentonita
dc.titleNew mycotoxin adsorbents based on tri-octahedral bentonites for animal feed
dc.typeinfo:eu-repo/semantics/article
dc.date.updated2020-01-20T12:04:18Z
dc.identifier.idgrec029462
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.identifier.doihttps://doi.org/10.1016/j.anifeedsci.2019.114228


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cc-by-nc-nd (c) Elsevier, 2019
Except where otherwise noted, this item's license is described as cc-by-nc-nd (c) Elsevier, 2019