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dc.contributor.authorPopova, Milka
dc.contributor.authorGuyader, Jessie
dc.contributor.authorSilberberg, Mathieu
dc.contributor.authorSeradj, Ahmad Reza
dc.contributor.authorSaro, Cristina
dc.contributor.authorBernard, Aurélien
dc.contributor.authorGérard, Christine
dc.contributor.authorMartin, Cécile
dc.contributor.authorMorgavi, Diego P.
dc.date.accessioned2019-12-13T09:22:20Z
dc.date.available2019-12-13T09:22:20Z
dc.date.issued2019-02-06
dc.identifier.issn0099-2240
dc.identifier.urihttp://hdl.handle.net/10459.1/67716
dc.description.abstractDietary supplementation with linseed, saponins, and nitrate is a promising methane mitigation strategy in ruminant production. Here, we aimed to assess the effects of these additives on the rumen microbiota in order to understand underlying microbial mechanisms of methane abatement. Two 2-by-2 factorial design studies were conducted simultaneously, which also allowed us to make a broadbased assessment of microbial responses. Eight nonlactating cows were fed diets supplemented with linseed or saponin in order to decrease hydrogen production and nitrate to affect hydrogen consumption; also, combinations of linseed plus nitrate or saponin plus nitrate were used to explore the interaction between dietary treatments. Previous work assessed effects on methane and fermentation patterns. Rumen microbes were studied by sequencing 18S and 16S rRNA genes and ITS1 amplicons. Methanogen activity was monitored by following changes in mcrA transcript abundance. Nitrate fed alone or in combination in both studies dramatically affected the composition and structure of rumen microbiota, although impacts were more evident in one of the studies. Linseed moderately modified only bacterial community structure. Indicator operational taxonomic unit (OTU) analysis revealed that both linseed and nitrate reduced the relative abundance of hydrogen-producing Ruminococcaceae. Linseed increased the proportion of bacteria known to reduce succinate to propionate, whereas nitrate supplementation increased nitrate-reducing bacteria and decreased the metabolic activity of rumen methanogens. Saponins had no effect on the microbiota. Inconsistency found between the two studies with nitrate supplementation could be explained by changes in microbial ecosystem functioning rather than changes in microbial community structure.
dc.description.sponsorshipJ.G. was the recipient of an INRA-Région Auvergne Ph.D. scholarship. C.S. acknowledges receipt of a postdoctoral fellowship from Fundación Alfonso Martín Escudero (Madrid, Spain). We also thank NEOVIA for financial support.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherAmerican Society for Microbiology
dc.relation.isformatofVersió postprint del document publicat a: https://doi.org/10.1128/AEM.02657-18
dc.relation.ispartofApplied and Environmental Microbiology, 2019, vol. 85, num. 4
dc.rights(c) American Society for Microbiology, 2019
dc.subjectLinseed
dc.subjectMethane
dc.subjectMicrobiota
dc.subjectNitrate
dc.subjectRumen
dc.subjectSaponin
dc.titleChanges in the rumen microbiota of cows in response to dietary supplementation with nitrate, linseed, and saponin alone or in combination
dc.typeinfo:eu-repo/semantics/article
dc.date.updated2019-12-13T09:22:20Z
dc.identifier.idgrec028954
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.identifier.doihttps://doi.org/10.1128/AEM.02657-18


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