Universitat de Lleida
    • English
    • català
    • español
  • English 
    • English
    • català
    • español
  • Login
Repositori Obert UdL
View Item 
  •   Home
  • Recerca
  • Medicina Experimental
  • Articles publicats (Medicina Experimental)
  • View Item
  •   Home
  • Recerca
  • Medicina Experimental
  • Articles publicats (Medicina Experimental)
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Sprouty1 controls genitourinary development via its N-terminal tyrosine

Thumbnail
View/Open
028598.pdf (3.179Mb)
Issue date
2019-08-01
Author
Vaquero, Marta
Cuesta, Sara
Anerillas, Carlos
Altés, Gisela
Ribera i Calvet, Joan
Basson, M. Albert
Licht, Jonathan D.
Egea Navarro, Joaquim
Encinas Martín, Mario
Suggested citation
Vaquero, Marta; Cuesta, Sara; Anerillas, Carlos; Altés, Gisela; Ribera i Calvet, Joan; Basson, M. Albert; ... Encinas Martín, Mario. (2019) . Sprouty1 controls genitourinary development via its N-terminal tyrosine. Journal of the American Society of Nephrology, 2019, vol. 30, núm. 8, p. 1398-1411. https://doi.org/10.1681/ASN.2018111085.
Impact


Web of Science logo    citations in Web of Science

Scopus logo    citations in Scopus

Google Scholar logo  Google Scholar
Share
Export to Mendeley
Metadata
Show full item record
Abstract
Background: Congenital anomalies of the kidney and urinary tract (CAKUT) is a group of diseases that include a broad spectrum of developmental defects of the genitourinary system. Mouse models indicate that perturbations of the GDNF-Ret signaling pathway are a major genetic cause of CAKUT. Sprouty1 is an intracellular Ret inhibitor whose mutation results in supernumerary kidneys, megaureters, and hydronephrosis in mice. Both the molecular mechanisms and the structural domains critical for Sprouty function are a matter of controversy, partly because studies pursuing this objective rely on ectopic overexpression in cell lines. A conserved N-terminal tyrosine has been frequently, but not always, identified as critical for their function in vitro. Methods: We have generated Sprouty1 knockin mice bearing a tyrosine-to-alanine substitution in position 53, corresponding to the conserved N-terminal tyrosine of Sprouty1. We have characterized development of the genitourinary systems of these mice via different methods, including the use of reporter mice expressing EGFP form the Ret locus, and whole mount cytokeratin staining. Results: Mice lacking this tyrosine grow ectopic ureteric buds that ultimately will form supernumerary kidneys, a phenotype indistinguishable to that of Sprouty1 knockout mice. Sprouty1 knockin mice also present megaureters and vesicoureteral reflux, caused by failure of ureters to separate from Wolffian ducts and migrate to their definitive position. Conclusions: Tyrosine 53 is absolutely necessary to convey Sprouty1 function during genitourinary development.
URI
http://hdl.handle.net/10459.1/67681
DOI
https://doi.org/10.1681/ASN.2018111085
Is part of
Journal of the American Society of Nephrology, 2019, vol. 30, núm. 8, p. 1398-1411
European research projects
Collections
  • Publicacions de projectes de recerca del Plan Nacional [2223]
  • Articles publicats (IRBLleida) [770]
  • Articles publicats (Ciències Mèdiques Bàsiques) [479]
  • Articles publicats (Medicina Experimental) [252]

Related items

Showing items related by title, author, creator and subject.

  • Sprouty1 haploinsufficiency accelerates pheochromocytoma development in Pten+/- mice 

    Vaquero, Marta; Macià Armengol, Anna; Anerillas Aljama, Diego; Velasco Sánchez, Ana; Matias-Guiu, Xavier; Ribera i Calvet, Joan; Encinas Martín, Mario (Society for Endocrinology, 2016-02-01)
    Pheochromocytomas (PCCs) are rare catecholamine-secreting tumors arising from the chromaffin cells in the adrenal medulla. Closely related paragangliomas share developmental origin but arise extra-adrenally, in the paraganglia ...
  • 7-dehydrocholesterol efficiently supports Ret signaling in a mouse model of Smith-Opitz-Lemli syndrome 

    Gou Fàbregas, Myriam; Macià Armengol, Anna; Anerillas, Carlos; Vaquero, Marta; Jové Font, Mariona; Jain, Sanjay; Ribera i Calvet, Joan; Encinas Martín, Mario (Nature Publishing Group, 2016)
    Smith-Lemli-Opitz syndrome (SLOS) is a rare disorder of cholesterol synthesis. Affected individuals exhibit growth failure, intellectual disability and a broad spectrum of developmental malformations. Among them, renal ...
  • Barley β-glucan accelerates wound healing by favoring migration versus proliferation of human dermal fibroblasts 

    Pérez Fusté, Noel; Guasch Vallés, Marta; Guillén, Pere; Anerillas, Carlos; Cemeli, Tània; Pedraza González, Neus; Ferrezuelo, Francisco; Encinas Martín, Mario; Moralejo Vidal, Mª Angeles; Garí Marsol, Eloi (Elsevier, 2019)
    β-Glucans are considered candidates for the medication in different human pathologies. In this work, we have purified β-glucan from a selected barley line and tested their effects in primary human dermal fibroblasts. ...

Contact Us | Send Feedback | Legal Notice
© 2021 BiD. Universitat de Lleida
Metadata subjected to 
 

 

Browse

All of the repositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

Statistics

View Usage Statistics

D'interès

Política institucional d'accés obertDiposita les teves publicacionsDiposita dades de recercaSuport a la recerca

Contact Us | Send Feedback | Legal Notice
© 2021 BiD. Universitat de Lleida
Metadata subjected to