Recognition motifs rather than phylogenetic origin influence the ability of targeting peptides to import nuclear-encoded recombinant proteins into rice mitochondria
MetadataShow full item record
Mitochondria fulfil essential functions in respiration and metabolism as well as regulating stress responses and apoptosis. Most native mitochondrial proteins are encoded by nuclear genes and are imported into mitochondria via one of several receptors that recognize N-terminal signal peptides. The targeting of recombinant proteins to mitochondria therefore requires the presence of an appropriate N-terminal peptide, but little is known about mitochondrial import in monocotyledonous plants such as rice (Oryza sativa). To gain insight into this phenomenon, we targeted nuclear-encoded enhanced green fluorescent protein (eGFP) to rice mitochondria using six mitochondrial pre-sequences with diverse phylogenetic origins, and investigated their effectiveness by immunoblot analysis as well as confocal and electron microscopy. We found that the ATPA and COX4 (Saccharomyces cerevisiae), SU9 (Neurospora crassa), pFA (Arabidopsis thaliana) and OsSCSb (Oryza sativa) peptides successfully directed most of the eGFP to the mitochondria, whereas the MTS2 peptide (Nicotiana plumbaginifolia) showed little or no evidence of targeting ability even though it is a native plant sequence. Our data therefore indicate that the presence of particular recognition motifs may be required for mitochondrial targeting, whereas the phylogenetic origin of the pre-sequences probably does not play a key role in the success of mitochondrial targeting in dedifferentiated rice callus and plants.
Is part ofTransgenic Research, 2020, vol. 29, p. 37-52
European research projects
Showing items related by title, author, creator and subject.
Transit Peptides From Photosynthesis-Related Proteins Mediate Import of a Marker Protein Into Different Plastid Types and Within Different Species Eseverri, Álvaro; Baysal, Can; Medina Piles, Vicente; Capell Capell, Teresa; Christou, Paul; Rubio, Luis; Caro, Elena (Frontiers Media, 2020-09-25)Nucleus-encoded plastid proteins are synthesized as precursors with N-terminal targeting signals called transit peptides (TPs), which mediate interactions with the translocon complexes at the outer (TOC) and inner (TIC) ...
RNA2‐encoded VP37 protein of Broad bean wilt virus 1 is a determinant of pathogenicity, host susceptibility, and a suppressor of post‐transcriptional gene silencing Carpino, Caterina; Ferriol Safont, Inmaculada; Elvira-González, Laura; Medina Piles, Vicente; Rubio, Luis; Peri, Ezio; Davino, Salvatore; Galipienso Torregrosa, Luis (British Society for Plant PathologyJohn Wiley & Sons Ltd, 2020-09-16)Broad bean wilt virus 1 (BBWV‐1, genus Fabavirus, family Secoviridae) is a bipartite, single‐stranded positive‐sense RNA virus infecting many horticultural and ornamental crops worldwide. RNA1 encodes proteins involved in ...
Bottlenecks in carotenoid biosynthesis and accumulation in rice endosperm are influenced by the precursor–product balance Bai, Chao; Capell Capell, Teresa; Berman Quintana, Judit; Medina Piles, Vicente; Sandmann, Gerhard; Christou, Paul; Zhu, Changfu (Wiley, 2016)The profile of secondary metabolites in plants reflects the balance of biosynthesis, degradation and storage, including the availability of precursors and products that affect the metabolic equilibrium. We investigated the ...