Show simple item record

dc.contributor.authorRojano-Delgado, Antonia M.
dc.contributor.authorPortugal, João M.
dc.contributor.authorPalma-Bautista, Candelario
dc.contributor.authorAlcántara-de la Cruz, Ricardo
dc.contributor.authorTorra Farré, Joel
dc.contributor.authorAlcántara, Esteban
dc.contributor.authorPrado, Rafael de
dc.description.abstractEuphorbia heterophylla is a weed species that invades extensive crop areas in subtropical regions of Brazil. This species was previously controlled by imazamox, but the continuous use of this herbicide has selected for resistant biotypes. Two biotypes of E. heterophylla from southern Brazil, one resistant (R) and one susceptible (S) to imazamox, were compared. The resistance of the R biotype was confirmed by dose-response assays since it required 1250.2 g ai ha−1 to reduce the fresh weight by 50% versus 7.4 g ai ha−1 for the S biotype. The acetolactate synthase (ALS) enzyme activity was studied using ALS-inhibiting herbicides from five different chemical families. The R biotype required the highest concentrations to reduce this enzyme activity by 50%. A Ser653Asn mutation was found in the ALS gene of the R biotype. The experiments carried out showed that imazamox absorption and metabolism were not involved in resistance. However, greater 14C-imazamox root exudation was found in the R biotype (~70% of the total absorbed imazamox). Target site mutation in the ALS gene is the principal mechanism that explains the imazamox resistance of the R biotype, but root exudation seems to also contribute to the resistance of this biotype.
dc.description.sponsorshipThis work was funded by the AGL2016-78944-R (Spain) project and Asociación de Agroquímicos y Medioambiente.
dc.publisherSpringer Nature
dc.relation.isformatofReproducció del document publicat a:
dc.relation.ispartofScientific Reports, 2019, vol. 9, article num. 15423
dc.rightscc-by (c) Rojano-Delgado, Antonia M. et al., 2019
dc.subjectResistència a insecticides
dc.subjectControl de plagues
dc.titleTarget site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype

Files in this item


This item appears in the following Collection(s)

Show simple item record

cc-by (c) Rojano-Delgado, Antonia M. et al., 2019
Except where otherwise noted, this item's license is described as cc-by (c) Rojano-Delgado, Antonia M. et al., 2019