Modulatory effect of protein and carotene dietary levels on pig gut microbiota
Ramayo Caldas, Yuliaxis
MetadataShow full item record
In this study we investigated the impact of dietary protein and carotene levels on microbial functions and composition during the last month of purebred fattening Duroc pigs. Fecal microbiota was characterized using 16S ribosomal RNA sequencing at two points of live, 165 (T1) and 195 (T2) days. From 70 to 165 days of age, 32 pigs were divided into two groups fed either a standard-protein (SP) or a low-protein (LP) diet. In the last month (165-195 days), all pigs received a LP diet, either carotene- enriched (CE) or not (NC). Significant differences were observed between T1 and T2 at Amplicon Sequences Variants (ASVs), phylum and genus levels. In T1 group, Prevotella, Faecalibacterium and Treponema were the genera most influenced by dietary protein, together with predicted functions related with the degradation of protein. In contrast, the CE diet did not impact the microbiome diversity, although 160 ASVs were differentially abundant between CE and NC groups at T2. Weak stability of enterotype clusters across time-points was observed as consequence of medium-term dietary interventions. Our results suggest that during the last month of fattening, dietary protein have a stronger effect than carotenes on the modulation of the compositional and functional structure of the pig microbiota.
Is part ofScientific Reports, 2019, vol. 9, num. 1, p. 14582
European research projects
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as cc-by (c) González-Prendes, Rayner et al., 2019
Showing items related by title, author, creator and subject.
Linoleic acid metabolic pathway allows for an efficient increase of intramuscular fat content in pigs Gol, Sofia; González Prendes, Rayner; Bosch, Lluís; Tor i Naudí, Marc; Reixach, Josep; Pena i Subirà, Ramona Natacha; Estany Illa, Joan (BMC (part of Springer Nature), 2019-05-07)Background: Intramuscular fat (IMF) content is a relevant trait for high-quality meat products such as dry-cured ham, but increasing IMF has the undesirable correlated effect of decreasing lean growth. Thus, there is a ...
About the existence of common determinants of gene expression in the porcine liver and skeletal muscle González Prendes, Rayner; Mármol-Sánchez, Emilio; Quintanilla Aguado, Raquel; Castelló, Anna; Zidi, A.; Ramayo Caldas, Yuliaxis; Figueiredo Cardoso, Tainã; Manunza, Arianna; Cánovas, Angela; Amills i Eras, Marcel (BMC (part of Springer Nature), 2019-06-24)Background The comparison of expression QTL (eQTL) maps obtained in different tissues is an essential step to understand how gene expression is genetically regulated in a context-dependent manner. In the current work, we ...
Co-expression network analysis predicts a key role of microRNAs in the adaptation of the porcine skeletal muscle to nutrient supply Mármol-Sánchez, Emilio; Ramayo Caldas, Yuliaxis; Quintanilla Aguado, Raquel; Figueiredo Cardoso, Tainã; González Prendes, Rayner; Tibau, Joan; Amills i Eras, Marcel (BMC (part of Springer Nature), 2020-01-17)Background The role of non-coding RNAs in the porcine muscle metabolism is poorly understood, with few studies investigating their expression patterns in response to nutrient supply. Therefore, we aimed to investigate the ...