Susceptibility to organophosphate insecticides and activity of detoxifying enzymes in Spanish populations of Cydia pomonella (Lepidoptera: Tortricidae)
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The mechanisms associated with resistance of codling moth (Cydia pomonella (L.)) to organophosphate insecticides in pome fruit orchards have been shown to depend on the area. Our objectives were to evaluate the susceptibility of Spanish codling moth populations to chlorpyrifos-ethyl, azinphos-methyl and phosalone, and the activity of three enzymatic systems reported to be involved in resistance. Eleven field populations and a susceptible strain used as a reference were tested using a bioassay consisting in the topical application of a diagnostic concentration on post-diapausing larvae. The enzymatic activity of mixed-function oxidases (MFO), glutathione-S-transferases (GST) and esterases (EST) was measured in post-diapausing larvae and adults. A significant decrease in the efficacy of the organophosphates was observed for all field populations, though the decrease was smaller in the case of chlorpyrifos-ethyl. No differences between sexes were detected. In post-diapausing larvae, the activity of the three enzymatic systems was higher in all the field populations than in the susceptible one. The possible implication of EST in codling moth insecticide resistance is reported for the first time in European field populations. In adults, only MFO and GST were implicated. Codling moth resistance to organophosphates in Spanish populations must be taken into account in the implementation of anti-resistance strategies.
Is part ofJournal of Economic Entomology, 2010, vol. 103, num. 2, p. 482-491
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Resistance of Spanish codling moth (Cydia pomonella) populations to insecticides and activity of detoxifying enzymatic systems Rodríguez García, Marcela; Bosch Serra, Dolors; Avilla Hernández, Jesús (The Netherlands Entomological Society, 2011)Resistance of codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae) to insecticides has become a major problem in many apple and pear production areas. Our aim was to determine the level of insecticide resistance ...
Assessment of insecticide resistance in eggs and neonate larvae of Cydia pomonella (Lepidoptera: Tortricidae) Rodríguez García, Marcela; Marques, Tânia; Bosch Serra, Dolors; Avilla Hernández, Jesús (Elsevier, 2011)Spanish Cydia pomonella (L.) field populations have developed resistance to several insecticide groups. Diagnostic concentrations were established as the LC90 calculated on a susceptible strain (S_Spain) for five and seven ...
Determination of the baseline susceptibility of European populations of Cydia pomonella (Lepidoptera: Tortricidae) to chlorantraniliprole and the role of cytochrome P450 monooxygenases Bosch Serra, Dolors; Rodríguez García, Marcela; Depalo, Laura; Avilla Hernández, Jesús (Oxford University Press, 2018-02-01)The codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae), is the key pest on pome fruit and walnut orchards worldwide. Its resistance to available insecticides has been widely reported. Chlorantraniliprole is an ...