Multiple Resistance to Synthetic Auxin Herbicides and Glyphosate in Parthenium hysterophorus Occurring in Citrus Orchards

Mora, Andrés D.; Rosario, Jesús; Rojano-Delgado, Antonia M.; Palma-Bautista, Candelario; Torra Farré, Joel; Alcántara-de la Cruz, Ricardo; Prado, Rafael de

doi:https://doi.org/10.1021/acs.jafc.9b03988

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Issue date

2019-08-15

Author

Mora, Andrés D.

Rosario, Jesús

Rojano-Delgado, Antonia M.

Palma-Bautista, Candelario

Torra Farré, Joel

Alcántara-de la Cruz, Ricardo

Prado, Rafael de

Suggested citation

Mora, Andrés D.; Rosario, Jesús; Rojano-Delgado, Antonia M.; Palma-Bautista, Candelario; Torra Farré, Joel; Alcántara-de la Cruz, Ricardo; Prado, Rafael de; . (2019) . Multiple Resistance to Synthetic Auxin Herbicides and Glyphosate in Parthenium hysterophorus Occurring in Citrus Orchards. Journal of Agricultural and Food Chemistry, 2019, vol. 67, num. 36, p. 10010-10017. https://doi.org/10.1021/acs.jafc.9b03988.

Abstract

Dominican farmers have started to apply synthetic auxin herbicides (SAHs) as the main alternative to mitigate the impacts of the occurrence of glyphosate-resistant (GR) Parthenium hysterophorus populations in citrus orchards. A GR P. hysterophorus population survived field labeled rates of glyphosate, 2,4-dichlorophenoxyacetic acid (2,4-D), dicamba, and picloram, which showed poor control (<50%). In in vivo assays, resistance levels were high for glyphosate and moderate for picloram, dicamba, and 2,4-D. Sequencing the 5-enolpyruvylshikimate-3-phosphate synthase gene revealed the double Thr-102-Ile and Pro-106-Ser amino acid substitution, conferring resistance to glyphosate. Additionally, reduced absorption and impaired translocation contributed to this resistance. Regarding SAH, impaired 2,4-D transport and enhanced metabolism were confirmed in resistant plants. The application of malathion improved the efficacy of SAHs (control >50%), showing that metabolism of these herbicides was mediated by cytochrome P450 enzymes. This study reports, for the first time, multiple resistance to SAHs and glyphosate in P. hysterophorus.

URI

http://hdl.handle.net/10459.1/66698

DOI

https://doi.org/10.1021/acs.jafc.9b03988

Is part of

Journal of Agricultural and Food Chemistry, 2019, vol. 67, num. 36, p. 10010-10017