Comparison of the immunoperoxidase monolayer assay and three commercial ELISAs for detection of antibodies against porcine circovirus type 2

Pileri, Emanuela; Cortey, Martí; Rodríguez, Fernando; Sibila, Marina; Fraile Sauce, Lorenzo José; Segalés, Joaquim

doi:https://doi.org/10.1016/j.tvjl.2014.05.025

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Issue date

2014

Author

Pileri, Emanuela

Cortey, Martí

Rodríguez, Fernando

Sibila, Marina

Fraile Sauce, Lorenzo José

Segalés, Joaquim

Suggested citation

Pileri, Emanuela; Cortey, Martí; Rodríguez, Fernando; Sibila, Marina; Fraile Sauce, Lorenzo José; Segalés, Joaquim; . (2014) . Comparison of the immunoperoxidase monolayer assay and three commercial ELISAs for detection of antibodies against porcine circovirus type 2. Veterinary Journal, 2014, vol. 201, num. 3, p. 429-432. https://doi.org/10.1016/j.tvjl.2014.05.025.

Abstract

The aim of this study was to compare and correlate antibody titres against porcine circovirus type 2 (PCV2) in porcine sera (n = 1270) obtained by immunoperoxidase monolayer assay (IPMA) with the results of three commercial ELISAs (designated E1, E2 and E3). The correlation between IPMA and ELISA results was excellent (r2 ≥ 0.90). Compared to IPMA, E2 had the highest sensitivity (93.0%), followed by E3 (90.1%) and E1 (85.0%); the specificity was 100% for all tests. All three commercial ELISAs had predictive values similar to those of IPMA and could be used to monitor antibody responses against PCV2 infection and/or vaccination.

URI

http://hdl.handle.net/10459.1/66483

DOI

https://doi.org/10.1016/j.tvjl.2014.05.025

Is part of

Veterinary Journal, 2014, vol. 201, num. 3, p. 429-432

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Articles publicats (Agrotecnio Center) [1092]
Articles publicats (Ciència Animal) [615]

Except where otherwise noted, this item's license is described as cc-by-nc-nd, (c) Elsevier, 2014