Comparison of the immunoperoxidase monolayer assay and three commercial ELISAs for detection of antibodies against porcine circovirus type 2

Pileri, Emanuela; Cortey, Martí; Rodríguez, Fernando; Sibila, Marina; Fraile Sauce, Lorenzo José; Segalés, Joaquim

doi:https://doi.org/10.1016/j.tvjl.2014.05.025

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Issue date

2014

Author

Pileri, Emanuela

Cortey, Martí

Rodríguez, Fernando

Sibila, Marina

Fraile Sauce, Lorenzo José

Segalés, Joaquim

Abstract

The aim of this study was to compare and correlate antibody titres against porcine circovirus type 2 (PCV2) in porcine sera (n = 1270) obtained by immunoperoxidase monolayer assay (IPMA) with the results of three commercial ELISAs (designated E1, E2 and E3). The correlation between IPMA and ELISA results

was excellent (r2 ≥ 0.90). Compared to IPMA, E2 had the highest sensitivity (93.0%), followed by E3 (90.1%) and E1 (85.0%); the specificity was 100% for all tests. All three commercial ELISAs had predictive values similar to those of IPMA and could be used to monitor antibody responses against PCV2 infection and/or vaccination.

URI

http://hdl.handle.net/10459.1/66483

DOI

https://doi.org/10.1016/j.tvjl.2014.05.025

Is part of

Veterinary Journal, 2014, vol. 201, num. 3, p. 429-432

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Articles publicats (Agrotecnio Center) [677]
Articles publicats (Ciència Animal) [408]

Except where otherwise noted, this item's license is described as cc-by-nc-nd, (c) Elsevier, 2014