Comparison of the immunoperoxidase monolayer assay and three commercial ELISAs for detection of antibodies against porcine circovirus type 2

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2014Author
Pileri, Emanuela
Cortey, Martí
Rodríguez, Fernando
Sibila, Marina
Segalés, Joaquim
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Pileri, Emanuela;
Cortey, Martí;
Rodríguez, Fernando;
Sibila, Marina;
Fraile Sauce, Lorenzo José;
Segalés, Joaquim;
.
(2014)
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Comparison of the immunoperoxidase monolayer assay and three commercial ELISAs for detection of antibodies against porcine circovirus type 2.
Veterinary Journal, 2014, vol. 201, num. 3, p. 429-432.
https://doi.org/10.1016/j.tvjl.2014.05.025.
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The aim of this study was to compare and correlate antibody titres against porcine circovirus type 2 (PCV2) in porcine sera (n = 1270) obtained by immunoperoxidase monolayer assay (IPMA) with the results of three commercial ELISAs (designated E1, E2 and E3). The correlation between IPMA and ELISA results was excellent (r2 ≥ 0.90). Compared to IPMA, E2 had the highest sensitivity (93.0%), followed by E3 (90.1%) and E1 (85.0%); the specificity was 100% for all tests. All three commercial ELISAs had predictive values similar to those of IPMA and could be used to monitor antibody responses against PCV2 infection and/or vaccination.