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dc.contributor.authorDecourcelle, Mathilde
dc.contributor.authorPerez-Fons, Laura
dc.contributor.authorBaulande, Sylvain
dc.contributor.authorSteiger, Sabine
dc.contributor.authorCouvelard, Linhdavanh
dc.contributor.authorHem, Sonia
dc.contributor.authorZhu, Changfu
dc.contributor.authorCapell Capell, Teresa
dc.contributor.authorChristou, Paul
dc.contributor.authorFraser, Paul
dc.contributor.authorSandmann, Gerhard
dc.description.abstractThe aim of this study was to assess whether endosperm-specific carotenoid biosynthesis influenced core metabolic processes in maize embryo and endosperm and how global seed metabolism adapted to this expanded biosynthetic capacity. Although enhancement of carotenoid biosynthesis was targeted to the endosperm of maize kernels, a concurrent up-regulation of sterol and fatty acid biosynthesis in the embryo was measured. Targeted terpenoid analysis, and non-targeted metabolomic, proteomic, and transcriptomic profiling revealed changes especially in carbohydrate metabolism in the transgenic line. In-depth analysis of the data, including changes of metabolite pools and increased enzyme and transcript concentrations, gave a first insight into the metabolic variation precipitated by the higher up-stream metabolite demand by the extended biosynthesis capacities for terpenoids and fatty acids. An integrative model is put forward to explain the metabolic regulation for the increased provision of terpenoid and fatty acid precursors, particularly glyceraldehyde 3-phosphate and pyruvate or acetyl-CoA from imported fructose and glucose. The model was supported by higher activities of fructokinase, glucose 6-phosphate isomerase, and fructose 1,6-bisphosphate aldolase indicating a higher flux through the glycolytic pathway. Although pyruvate and acetyl-CoA utilization was higher in the engineered line, pyruvate kinase activity was lower. A sufficient provision of both metabolites may be supported by a by-pass in a reaction sequence involving phosphoenolpyruvate carboxylase, malate dehydrogenase, and malic enzyme.ca_ES
dc.description.sponsorshipFunding through the Plant KBBE project CaroMaize is gratefully acknowledged. PDF and LP are grateful for funding from the EU FP7 project DISCO grant number 613513.ca_ES
dc.publisherOxford University Pressca_ES
dc.relation.isformatofReproducció del document publicat a
dc.relation.ispartofJournal of Experimental Botany, 2015, vol. 66, núm. 11, p. 3141–3150ca_ES
dc.rightscc-by, (c) Decourcelle et al., 2015ca_ES
dc.subjectGenetically engineered carotenoid biosynthesisca_ES
dc.subjectGM maizeca_ES
dc.subjectPathway regulationca_ES
dc.titleCombined transcript, proteome, and metabolite analysis of transgenic maize seeds engineered for enhanced carotenoid synthesis reveals pleotropic effects in core metabolismca_ES

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cc-by, (c) Decourcelle et al., 2015
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