Digestive microbiota is different in pigs receiving antimicrobials or a feed additive during the nursery period

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2018Author
Soler, Cassandra
Goossens, Tim
Bermejo, Alvaro
Migura Garcia, Lourdes
Cusco, Anna
Francino, Olga
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Soler, Cassandra;
Goossens, Tim;
Bermejo, Alvaro;
Migura Garcia, Lourdes;
Cusco, Anna;
Francino, Olga;
Fraile Sauce, Lorenzo José;
.
(2018)
.
Digestive microbiota is different in pigs receiving antimicrobials or a feed additive during the nursery period.
PLoS ONE, 2018, vol.13, núm.5, e0197353.
https://doi.org/10.1371/journal.pone.0197353.
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Antimicrobials have been used in a prophylactic way to decrease the incidence of digestive
disorders during the piglet post-weaning period. Nowadays, it is urgent to reduce their consumption
in livestock to address the problem of antimicrobial resistance. In this study, the
effect of a product on piglet microbiota has been investigated as an alternative to antimicrobials.
Three groups of ten post-weaning pigs were sampled at 0, 15 and 30 days one week
post-weaning; the control, antibiotic and feed additive group received a standard post-weaning
diet without antibiotics or additives, the same diet as the control group but with amoxicillin
and colistin sulphate and the same diet as the control group but with a feed additive
(Sanacore-EN, Nutriad International N.V.), respectively. The total DNA extracted from faeces
was used to amplify the 16S RNA gene for massive sequencing under manufacturer's
conditions. Sequencing data was quality filtered and analyzed using QIIME software and
suitable statistical methods. In general terms, age modifies significantly the microbiota of
the piglets. Thus, the oldest the animal, the highest bacterial diversity observed for the control
and the feed additive groups. However, this diversity was very similar in the antibiotic
group throughout the trial. Interestingly, a clear increase in abundance of Bacillus and Lactobacillus
spp was detected within the feed additive group versus the antibiotic and control
groups. In conclusion, the feed additive group had a positive effect in the endogenous microbiota
of post-weaning pigs increasing both, the diversity of bacterial families and the abundance
of lactic acid bacteria during the post-weaning period.
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PLoS ONE, 2018, vol.13, núm.5, e0197353European research projects
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