Unravelling the contribution of the Penicillium expansum PeSte12 transcription factor to virulence during apple fruit infection
Torres Sanchis, Rosario
MetadataShow full item record
Blue mould disease caused by Penicillium expansum infection is one of the most important diseases of pome fruit accounting for important economic losses. In the present study, the PeSte12 transcription factor gene was identified, and deletant mutants were produced by gene replacement. Knockout mutants showed a significant decrease of virulence during apple fruit infection. Virulence was affected by the maturity stage of the fruit (immature, mature and over-mature), and disease severity was notably reduced when the apples were stored at 0 °C. The ΔPeSte12 mutants resulted defective in asexual reproduction, producing less conidia, but this characteristic did not correlate with differences in microscopic morphology. In addition, the ΔPeSte12 mutants produced higher quantity of hydrogen peroxide than the wild type strain. Gene expression analysis revealed that PeSte12 was induced over time during apple infection compared to axenic growth, particularly from 2 dpi, reinforcing its role in virulence. Analysis of transcriptional abundance of several genes in ΔPeSte12 mutants showed that in most of the evaluated genes, PeSte12 seemed to act as a negative regulator during axenic growth, as most of them exhibited an increasing expression pattern along the time period evaluated. The highest expression values corresponded to detoxification, ATPase activity, protein folding and basic metabolism. Gene expression analysis during apple infection showed that 3 out of 9 analysed genes were up regulated; thus, PeSte12 seemed to exert a positive control to particular type of aldolase. These results demonstrate the PeSte12 transcription factor could play an important role in P. expansum's virulence and asexual reproduction.
Is part ofFood Microbiology, 2018, vol. 69, p. 123-135
European research projects
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Differential contribution of the two major polygalacturonases from Penicillium digitatum to virulence towards citrus fruit Vilanova Torren, Laura; López-Pérez, M.; Ballester, Ana-Rosa; Teixidó i Espasa, Neus; Usall i Rodié, Josep; Lara Ayala, Isabel; Viñas Almenar, Inmaculada; Torres Sanchis, Rosario; González-Candelas, Luis (Elsevier, 2018-10)The fungus Penicillium digitatum is the causal agent of the citrus green mould, the major postharvest diseases of citrus fruit. Lesions on the surface of infected fruits first appear as soft areas around the inoculation ...
Relevance of the transcription factor PdSte12 in Penicillium digitatum conidiation and virulence during citrus fruit infection Vilanova, Laura; Teixidó i Espasa, Neus; Torres Sanchis, Rosario; Usall i Rodié, Josep; Viñas Almenar, Inmaculada; Sánchez-Torres, Paloma (Elsevier B.V., 2016)Green mould, resulting from Penicillium digitatum, is the most important postharvest disease of citrus. In a previous study, the PdSte12 transcription factor gene was identified, and disruption mutants were obtained. In ...
Effect of fruit maturity stage of orange on the wound response to Penicillium digitatum (pathogen) and P. expansum (non-host pathogen) Vilanova, Laura; Torres Sanchis, Rosario; Usall i Rodié, Josep; Teixidó i Espasa, Neus; González-Candelas, Luis; Viñas Almenar, Inmaculada; Fiori, S. (International Society for Horticultural Science, 2014)Penicillium digitatum and P. expansum are postharvest necrotrophic fungi that require wounds to infect the fruit. Therefore, injuries caused during harvesting and postharvest handling provide an optimal locus for infection. ...