Barley β-glucan accelerates wound healing by favoring migration versus proliferation of human dermal fibroblasts
Pérez Fusté, Noel
Guasch Vallés, Marta
MetadataShow full item record
β-Glucans are considered candidates for the medication in different human pathologies. In this work, we have purified β-glucan from a selected barley line and tested their effects in primary human dermal fibroblasts. Unexpectedly, we have observed that this compound promoted a short-transitory proliferation
arrest at 24 h after its addition on the medium. We have determined that this transitory arrest was dependent on the cell-cycle regulator protein Retinoblastoma. Moreover, dermal fibroblasts increase their migration capacities at 24 h after barley β-glucan addition. Also, we have described that barley β-glucan strongly reduced the ability of fibroblasts to attach and to spread on cell plates. Our data indicates that barley β-glucan signal induces an early response in HDF cells favoring migration versus proliferation. This feature is consistent with our observation that the topical addition of our barley β-glucan in vivo accelerates the wound closure in mouse skin.
Is part ofCarbohydrate Polymers, 2019, vol. 210, p. 389-398
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Cemeli, Tània; Guasch Vallés, Marta; Nàger Grifo, Mireia; Felip, Isidre; Cambray Carner, Serafí; Santacana Espasa, Maria; Gatius Calderó, Sònia; Pedraza González, Neus; Dolcet Roca, Xavier; Ferrezuelo, Francisco; Schuhmacher, Alberto J.; Herreros Danés, Judit; Garí Marsol, Eloi (Wiley, 2019)Glioblastoma (GBM) is a highly invasive brain neoplasia with an elevated recurrence rate after surgical resection. The CyclinD1 (Ccnd1)/Cdk4-RB1 axis is frequently altered in GBM, leading to over-proliferation by RB1 ...
Cytoplasmic cyclin D1 regulates cell invasion and metastasis through the phosphorylation of paxillin Pérez Fusté, Noel; Fernández Hernández, Rita; Cemeli, Tània; Mirantes Barbeito, Cristina; Pedraza González, Neus; Rafel i Borrell, Marta; Torres Rosell, Jordi; Colomina i Gabarrella, Neus; Ferrezuelo, Francisco; Dolcet Roca, Xavier; Garí Marsol, Eloi (Nature Publishing Group, 2016)Cyclin D1 (Ccnd1) together with its binding partner Cdk4 act as a transcriptional regulator to control cell proliferation and migration, and abnormal Ccnd1 Cdk4 expression promotes tumour growth and metastasis. While ...
Pérez Fusté, Noel; Castelblanco Echavarría, Esmeralda; Felip, Isidre; Santacana Espasa, Maria; Fernández-Hernández, Rita; Gatius Calderó, Sònia; Pedraza González, Neus; Pallares, Judit; Cemeli, Tània; Valls Marsal, Joan; Tarres, Marc; Ferrezuelo, Francisco; Dolcet Roca, Xavier; Matias-Guiu, Xavier; Garí Marsol, Eloi (Impact Journals, 2016)Cyclin D1 (Ccnd1) is a proto-oncogen amplified in many different cancers and nuclear accumulation of Ccnd1 is a characteristic of tumor cells. Ccnd1 activates the transcription of a large set of genes involved in cell ...