Faecal bacterial and short-chain fatty acids signature in hypercholesterolemia

Granado-Serrano, Ana Belén; Martín Garí, Meritxell; Sánchez, V.; Riart Solans, M.; Berdún Hernández, Rebeca; Ludwig, Iziar A.; Rubió Piqué, Laura; Vilaprinyo Terré, Ester; Portero Otín, Manuel; Serrano Casasola, José Carlos Enrique

doi:https://doi.org/10.1038/s41598-019-38874-3

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2019

Author

Granado-Serrano, Ana Belén

Martín Garí, Meritxell

Sánchez, V.

Riart Solans, M.

Berdún Hernández, Rebeca

Ludwig, Iziar A.

Rubió Piqué, Laura

Vilaprinyo Terré, Ester

Portero Otín, Manuel

Serrano Casasola, José Carlos Enrique

Suggested citation

Granado-Serrano, Ana Belén; Martín Garí, Meritxell; Sánchez, V.; Riart Solans, M.; Berdún Hernández, Rebeca; Ludwig, Iziar A.; ... Serrano Casasola, José Carlos Enrique. (2019) . Faecal bacterial and short-chain fatty acids signature in hypercholesterolemia. Scientific Reports, 2019, vol. 9, núm. 1772, p. 1-13. https://doi.org/10.1038/s41598-019-38874-3.

Abstract

Gut microbiota has been suggested to affect lipid metabolism. The objective of this study was to characterize the faecal microbiota signature and both short chain fatty acids (SCFAs) and bile acids (BA) profile of hypercholesterolemic subjects. Microbiota composition, SCFAs, BA and blood lipid profile from male volunteers with hypercholesterolemia (HC) and normocholesterolemia (NC) were determined by 16S rDNA sequencing, HPLC, GC and NMR, respectively. HC subjects were characterized by having lower relative abundance of Anaeroplasma (0.002% vs 0.219%, p-value = 0.026) and Haemophilus (0.041% vs 0.078%, p-value = 0.049), and higher of Odoribacter (0.51% vs 0.16%; p-value = 0.044). Correlation analysis revealed that Anaeroplasma and Haemophilus were associated to an unfavourable lipid profile: they correlated negatively to cholesterol and triglycerides related biomarkers and the ratio total to high density lipoprotein (HDL) cholesterol, and positively to HDL size. Odoribacter displayed an opposite behaviour. Faecal SCFAs profile revealed higher abundance of isobutyric (2.76% vs 0.82%, p-value = 0.049) and isovaleric acid (1.32% vs 0.06%, p-value = 0.016) in HC. Isobutyric acid correlated positively with Odoribacter and lipid parameters indicative of an unfavourable profile. BA profile did not show differences between groups. It was concluded that HC subjects showed a particular faecal bacterial signature and SCFAs profile associated with their lipid profile.

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http://hdl.handle.net/10459.1/65826

DOI

https://doi.org/10.1038/s41598-019-38874-3

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Scientific Reports, 2019, vol. 9, núm. 1772, p. 1-13

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Except where otherwise noted, this item's license is described as cc-by (c) Granado-Serrano, Ana Belén et al., 2019