Whi3, a developmental regulator of budding yeast, binds a large set of mRNAs functionally related to the endoplasmic reticulum
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Whi3 is an RNA-binding protein associated with the endoplasmic reticulum (ER) that binds the CLN3 mRNA and plays a key role in the efficient retention of cyclin Cln3 at the ER. In the present work, we have identified new Whi3-associated mRNAs by a genomic approach. A large and significant number of these Whi3 targets encode for membrane and exocytic proteins involved in processes such as transport and cell wall biogenesis. Consistent with the genomic data, we have observed that cell wall integrity is compromised in Whi3-deficient cells and found strong genetic interactions between WHI3 and the cell integrity pathway. Whi3-associated mRNAs are enriched in clusters of the tetranucleotide GCAU, and mutation of the GCAU clusters in the CLN3 mRNA caused a reduction in its association to Whi3, suggesting that these sequences may act as cis-determinants for binding. Our data suggest that Whi3 is involved in the regulation and/or localization of a large subset of mRNAs functionally related to the ER and, since it is important for different molecular processes such as cytoplasmic retention or exocytic traffic of proteins, we propose that Whi3 is a general modulator of protein fate in budding yeast.
Is part ofJournal of Biological Chemistry, 2008, vol. 283, núm. 42, p. 28670-9
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Colomina i Gabarrella, Neus; Ferrezuelo, Francisco; Vergés, Emili; Aldea, Martí; Garí Marsol, Eloi (Landes Bioscience, 2009)The Whi3 protein is associated with the endoplasmic reticulum, interacts with Cdc28, the budding-yeast Cdk, binds the mRNA of cyclin CLN3 and prevents accumulation of the Cdc28-Cln3 in the nucleus until late G1. Besides ...
The critical size is set at a single-cell level by growth rate to attain homeostasis and adaptation Ferrezuelo, Francisco; Colomina i Gabarrella, Neus; Palmisano, Alida; Garí Marsol, Eloi; Gallego, Carme; Csikász-Nagy, Attila; Aldea, Martí (Nature, 2012)Budding yeast cells are assumed to trigger Start and enter the cell cycle only after they attain a critical size set by external conditions. However, arguing against deterministic models of cell size control, cell volume ...
The transcriptional network activated by Cln3 cyclin at the G1-to-S transition of the yeast cell cycle Ferrezuelo, Francisco; Colomina i Gabarrella, Neus; Futcher, Bruce; Aldea, Martí (BioMed Central, 2010)Background: The G1-to-S transition of the cell cycle in the yeast Saccharomyces cerevisiae involves an extensive transcriptional program driven by transcription factors SBF (Swi4-Swi6) and MBF (Mbp1-Swi6). Activation of ...