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dc.contributor.authorGarí Marsol, Eloi
dc.contributor.authorVolpe, Tom
dc.contributor.authorWang, Hongyin
dc.contributor.authorGallego, Carme
dc.contributor.authorFutcher, Bruce
dc.contributor.authorAldea, Martí
dc.date.accessioned2019-02-27T09:17:02Z
dc.date.available2019-02-27T09:17:02Z
dc.date.issued2001
dc.identifier.issn0890-9369
dc.identifier.urihttp://hdl.handle.net/10459.1/65801
dc.description.abstractEukaryotic cells commit in G1 to a new mitotic cycle or to diverse differentiation processes. Here we show that Whi3 is a negative regulator of Cln3, a G1 cyclin that promotes transcription of many genes to trigger the G1/S transition in budding yeast. Whi3 contains an RNA-recognition motif that specifically binds the CLN3 mRNA, with no obvious effects on Cln3 levels, and localizes the CLN3 mRNA into discrete cytoplasmic foci. This is the first indication that G1 events may be regulated by locally restricting the synthesis of a cyclin. Moreover, Whi3 is also required for restraining Cln3 function in meiosis, filamentation, and mating, thus playing a key role in cell fate determination in budding yeast.
dc.description.sponsorshipWe thank A. Bueno, C. Mann, S. Moreno, E. Schwob, and B. Séraphin for strains and helpful discussions, and J.X. Comella for comments on the manuscript. This work was supported by grants from the Spanish Ministry of Education and Culture, and La Paeria to E.G. and M.A., from AECI to H.W. and from the NIH to B.F. and T.V.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherCold Spring Harbor Laboratory Press
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1101/gad.203501
dc.relation.ispartofGenes & Development, 2001, vol. 15, núm. 21, p. 2803-8
dc.rights(c) Garí Marsol, Eloi et al., 2001
dc.subjectCell cycle
dc.subjectG1 regulation
dc.subjectDifferentiation
dc.subjectRNA-binding protein
dc.subjectCyclin
dc.subjectmRNA localization
dc.titleWhi3 binds the mRNA of the G(1) cyclin CLN3 to modulate cell fate in budding yeast
dc.typeinfo:eu-repo/semantics/article
dc.date.updated2019-02-27T09:17:02Z
dc.identifier.idgrec004082
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.identifier.doihttps://doi.org/10.1101/gad.203501


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