Abiotic factors and their interactions influence on the co-production of aflatoxin B1 and cyclopiazonic acid by Aspergillus flavus isolated from corn
MetadataShow full item record
The objectives of this study were i) to determine the effects of the interactions of water activity, temperature and incubation time on the co-production of AFB1 and CPA by isolates of Aspergillus flavus with different profile of mycotoxin production and ii) to identify the aW and temperature limiting conditions for the production of both mycotoxins. Fungi used in this study were selected because they belonged to different chemotypes: chemotype I (AFB1/CPA), III (AFB1/CPA ) and IV (AFB1 /CPA), respectively. Two culture media were used; Czapek yeast agar (CYA) and corn extract agar (CEM), at different incubated temperatures (10-40º C) and aW levels (0.80-0.98). AFB1 and CPA production were analyzed after 7, 14, 21 and 28 days of incubation. Significant differences were observed with respect to mycotoxin production depending on the media evaluated. The AFB1 production occurred more favorably on CYA while the highest CPA concentrations were recorded on CEM. Within the range of aW evaluated in this study, 0.83 was the limiting level for both toxins production. The optimum conditions for AFB1 production occurred at 0.96 aw and 30 C after 21 days of incubation, regardless of the media and isolate. Although different amounts of toxins were produced in each medium, the limiting and optimum conditions for their production were similar in both. No differences in the response of the three isolates to the abiotic factors discussed were observed despite belonging to different chemotypes. The determination of the thresholds of mycotoxins co-production, especially in the case of data obtained with the corn extract medium can be useful to avoid the conditions conducive to co-occurrence of these mycotoxins in corn.
Is part ofFood Microbiology, 2014, vol. 38, p. 276-283
European research projects
Showing items related by title, author, creator and subject.
Modelling the Probability of Growth and Aflatoxin B1 Production of Aspergillus Flavus under Changing Temperature Conditions in Pistachio Nuts Aldars García, Laila; Ramos Girona, Antonio J.; Sanchís Almenar, Vicente; Marín Sillué, Sònia (Elsevier, 2016)The aim of this work was to use probability models for the prediction of growth and aflatoxin production by Aspergillus flavus as a strategy to mitigate the aflatoxin presence in pistachio nuts during postharvest. Logistic ...
An attempt to model the probability of growth and aflatoxin B1 production of Aspergillus flavus under non-isothermal conditions in pistachio nuts Aldars García, Laila; Ramos Girona, Antonio J.; Sanchís Almenar, Vicente; Marín Sillué, Sònia (Elsevier, 2015)Human exposure to aflatoxins in foods is of great concern. The aim of this work was to use predictive mycology as a strategy to mitigate the aflatoxin burden in pistachio nuts postharvest. The probability of growth and ...
Modeling kinetics of aflatoxin production by Aspergillus flavus in maize-based medium and maize grain García, Daiana; Ramos Girona, Antonio J.; Sanchís Almenar, Vicente; Marín Sillué, Sònia (Elsevier, 2013)Predictive mycology has dealt mainly with germination, growth and inactivation of fungi while the issue of mycotoxin production remains relatively unexplored. Very few studies provide biomass dry weight/colony size data ...