Low doses of ochratoxin A induce micronucleus formation and delay DNA repair in human lymphocytes

Thumbnail Image
González Arias, Cyndia A.
Benitez-Trinidad, alma B.
Sordo, Montserrat
Robledo-Marenco, Lourdes
Medina-Díaz, Irma M.
Barrón-Vivanco, Briscia S.
Marín Sillué, SòniaMarín Sillué, Sònia - ORCID ID
Sanchís Almenar, VicenteSanchís Almenar, Vicente - ORCID ID
Ramos Girona, Antonio J.Ramos Girona, Antonio J. - ORCID ID
Rojas-García, A. E.
Other authors
cc-by-nc-nd (c) Elsevier, 2014
Journal Title
Journal ISSN
Volume Title
The contamination of food commodities by fungal toxins has attracted great interest because many of these mycotoxins are responsible for different diseases, including cancer and other chronic illnesses. Ochratoxin A (OTA) is a mycotoxin naturally present in food, and long-term exposure to food contaminated with low levels of OTA has been associated with renal cancer. In the present study, the cytotoxicity, cytostaticity, and genotoxicity of OTA (0.075-15 μM) in human lymphocytes were evaluated. A comet assay, a modified comet assay (DNA repair assay), which uses N-hydroxyurea (NHU) to detect nonrepaired lesions produced by OTA, and a cytokinesis-blocked micronucleus assay were used. Treatments with OTA were not cytotoxic, but OTA caused a cytostatic effect in human lymphocytes at a concentration of 15 μM. OTA (0.075-5 μM) produced a slight increase in the percentage of DNA in the comets and a delay in the DNA repair capacity of the lymphocytes. Micronucleus (MN) induction was observed at OTA concentrations of 1.5 and 5 μM. Our results indicate that OTA induces DNA stable damage at low doses that are neither cytotoxic nor cytostatic, and OTA delays the DNA repair kinetics. These findings indicate that OTA affects two pivotal events in the carcinogenesis pathway.
Journal or Serie
Food and Chemical Toxicology, 2014, vol. 74, p. 249-254