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dc.contributor.authorGonzález Arias, Cyndia A.
dc.contributor.authorBenitez-Trinidad, alma B.
dc.contributor.authorSordo, Montserrat
dc.contributor.authorRobledo-Marenco, Lourdes
dc.contributor.authorMedina-Díaz, Irma M.
dc.contributor.authorBarrón-Vivanco, Briscia S.
dc.contributor.authorMarín Sillué, Sònia
dc.contributor.authorSanchís Almenar, Vicente
dc.contributor.authorRamos Girona, Antonio J.
dc.contributor.authorRojas-García, A. E.
dc.description.abstractThe contamination of food commodities by fungal toxins has attracted great interest because many of these mycotoxins are responsible for different diseases, including cancer and other chronic illnesses. Ochratoxin A (OTA) is a mycotoxin naturally present in food, and long-term exposure to food contaminated with low levels of OTA has been associated with renal cancer. In the present study, the cytotoxicity, cytostaticity, and genotoxicity of OTA (0.075-15 μM) in human lymphocytes were evaluated. A comet assay, a modified comet assay (DNA repair assay), which uses N-hydroxyurea (NHU) to detect nonrepaired lesions produced by OTA, and a cytokinesis-blocked micronucleus assay were used. Treatments with OTA were not cytotoxic, but OTA caused a cytostatic effect in human lymphocytes at a concentration of 15 μM. OTA (0.075-5 μM) produced a slight increase in the percentage of DNA in the comets and a delay in the DNA repair capacity of the lymphocytes. Micronucleus (MN) induction was observed at OTA concentrations of 1.5 and 5 μM. Our results indicate that OTA induces DNA stable damage at low doses that are neither cytotoxic nor cytostatic, and OTA delays the DNA repair kinetics. These findings indicate that OTA affects two pivotal events in the carcinogenesis pathway.
dc.description.sponsorshipThe authors are grateful to the Spanish (Project AGL2011-24862) and Catalonian (XaRTA-Reference Network on Food Technology) Governments for their financial support. C.A. González-Arias thanks the Secretaria de Universitats i Recerca del Departament de Economia i Coneixement of the Generalitat de Catalunya for the pre-doctoral grant. The authors also thank Q.F.B. Guillermina Vázquez Estrada, Francisco Alberto Verdín Betancourt, and Carlos Alberto Martínez Delgado for their technical assistance.
dc.relation.isformatofVersió postprint del document publicat a:
dc.relation.ispartofFood and Chemical Toxicology, 2014, vol. 74, p. 249-254
dc.rightscc-by-nc-nd (c) Elsevier, 2014
dc.subjectOcratoxina A
dc.subjectComet assay
dc.subjectCytokinesis-blocked micronucleus (CBMN) assay
dc.titleLow doses of ochratoxin A induce micronucleus formation and delay DNA repair in human lymphocytes

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cc-by-nc-nd (c) Elsevier, 2014
Except where otherwise noted, this item's license is described as cc-by-nc-nd (c) Elsevier, 2014