Low doses of ochratoxin A induce micronucleus formation and delay DNA repair in human lymphocytes
González Arias, Cyndia A.
Benitez-Trinidad, alma B.
Medina-Díaz, Irma M.
Barrón-Vivanco, Briscia S.
Rojas-García, A. E.
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The contamination of food commodities by fungal toxins has attracted great interest because many of these mycotoxins are responsible for different diseases, including cancer and other chronic illnesses. Ochratoxin A (OTA) is a mycotoxin naturally present in food, and long-term exposure to food contaminated with low levels of OTA has been associated with renal cancer. In the present study, the cytotoxicity, cytostaticity, and genotoxicity of OTA (0.075-15 μM) in human lymphocytes were evaluated. A comet assay, a modified comet assay (DNA repair assay), which uses N-hydroxyurea (NHU) to detect nonrepaired lesions produced by OTA, and a cytokinesis-blocked micronucleus assay were used. Treatments with OTA were not cytotoxic, but OTA caused a cytostatic effect in human lymphocytes at a concentration of 15 μM. OTA (0.075-5 μM) produced a slight increase in the percentage of DNA in the comets and a delay in the DNA repair capacity of the lymphocytes. Micronucleus (MN) induction was observed at OTA concentrations of 1.5 and 5 μM. Our results indicate that OTA induces DNA stable damage at low doses that are neither cytotoxic nor cytostatic, and OTA delays the DNA repair kinetics. These findings indicate that OTA affects two pivotal events in the carcinogenesis pathway.
Is part ofFood and Chemical Toxicology, 2014, vol. 74, p. 249-254
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Modulation of the xenobiotic transformation system and inflammatory response by ochratoxin A exposure using a co-culture system of Caco-2 and HepG2 cells González Arias, Cyndia A.; Crespo Sempere, Ana; Marín Sillué, Sònia; Sanchís Almenar, Vicente; Ramos Girona, Antonio J. (Elsevier, 2015)Cytotoxicity of ochratoxin A (OTA) was evaluated using the MTS assay, and membrane integrity was measured using transepithelial electrical resistance (TEER). A transwell system was used to investigate the effect of OTA on ...
UPLC-MS/MS analysis of ochratoxin A metabolites produced by Caco-2 and HepG2 cells in a co-culture system González Arias, Cyndia A.; Marín Sillué, Sònia; Rojas-García, A. E.; Sanchís Almenar, Vicente; Ramos Girona, Antonio J. (Elsevier, 2017-09-19)Ochatoxin A (OTA) is one of the most important mycotoxins based on its toxicity. The oral route is the main gateway of entry of OTA into the human body, and specialized epithelial cells constitute the first barrier. The ...
González Arias, Cyndia A.; Piquer Garcia, I.; Marín Sillué, Sònia; Sanchís Almenar, Vicente; Ramos Girona, Antonio J. (Wageningen Academic Publishers, 2014)Ochratoxin A (OTA) is a mycotoxin produced by Aspergillus and Penicillium species with immunosuppressive, teratogenic, and carcinogenic properties. It has been determined that wine is the second largest source of OTA (10% ...