A multicentre study investigating parameters which influence direct bacterial identification from urine
Bosch Mestres, Jordi
Liébana Martos, Carmen
Ruiz de Alegría, Carlos
MetadataShow full item record
Rapid diagnosis is one of the best ways to improve patient management and prognosis as well as to combat the development of bacterial resistance. The aim of this study was to study parameters that impact the achievement of reliable identification using a combination of flow cytometry and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF-MS).The study was carried out in nine hospitals in Spain and included 1,050 urine samples with bacterial counts of 5x106 bacteria/ml. MALDI-ToF-MS-based identification was performed according to a previously described protocol. Valid identification by direct MALDI-ToF-MS was obtained in 72.8% of samples, in 80.3% of samples found to be positive by culture, 32.2% of contaminated samples, and 19.7% of negative samples. Among the positives samples with a valid identification the concordance at the species level was 97.2%. The parameters related to success of direct identification were: high bacterial count, the presence of Escherichia coli as a pathogen and rod-bacteria morphology provided by flow cytometry. The parameters related to failure were a high epithelial cell (EC) count, a high white blood cell (WBC) count and urine samples obtained from in-patients. In summary, this multicentre study confirms previously published data on the usefulness and accuracy of direct MALDI-ToF-MS-based identification of bacteria from urine samples. It seems important to evaluate not only the bacterial count, but also other parameters, such as EC and WBC counts, bacterial species and morphology, and the health care setting, to decide whether the sample is suitable for direct identification.
Is part ofPLoS ONE, 2018, vol. 13, núm. 12, e0207822
European research projects
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as cc-by (c) Yuliya Zhoromyrska et al., 2018
Showing items related by title, author, creator and subject.
The effectiveness of influenza vaccination in preventing hospitalisations of elderly individuals in two influenza seasons: a multicentre case–control study, Spain, 2013/14 and 2014/15 Domínguez, Angela; Soldevila, Núria; Toledo, Diana; Godoy i García, Pere; Espejo, Elena; Fernandez, Maria Amelia; Mayoral, José María; Castilla, Jesús; Egurrola, Mikel; Tamames, Sonia; Astray, Jenaro; Morales, María (European Centre for Disease Prevention and Control, 2017)Influenza vaccination may limit the impact of influenza in the community. The aim of this study was to assess the effectiveness of influenza vaccination in preventing hospitalisation in individuals aged ≥ 65 years in Spain. ...
Genome-Wide Association Study of VKORC1 and CYP2C9 on acenocoumarol dose, stroke recurrence and intracranial haemorrhage in Spain Cullell, Natalia; Carrera, Caty; Muiño, Elena; Torres-Aguila, Nuria-Paz; Cárcel-Márquez, Jara; González-Sánchez, Jonathan; Gallego Fabrega, Cristina; Molina, Jessica; Besora, Sarah; Sotoca, Javier; Buongiorno, Maria-Teresa; Jiménez-Conde, Jordi; Giralt-Steinhauer, Eva; de Torres-Chacón, Reyes; Montaner, Joan; Mancha, Fernando; Cabezas, Juan A.; Martí Fàbregas, Joan; Prats Sánchez, Luis; Camps Renom, Pol; Purroy Garcia, Francisco; Cambray Carner, Serafí; Freijo, María del Mar; Vives-Bauzá, Cristòfol; Tur, Silvia; Font, Maria-Àngels; López-Cancio, Elena; Hernandez-Perez, Maria; Obach, Victor; Calleja, Ana; Arenillas, Juan; Rodríguez-Yáñez, Manuel; Castillo, José; Sobrino, Tomas; Fernández-Cádenas, Israel; Krupinski, Jerzy (Nature Publishing Group, 2020)Acenocoumarol is an oral anticoagulant with significant interindividual dose variations. Variants in CYP2C9 and VKORC1 have been associated with acenocoumarol maintenance dose. We analysed whether any of the 49 polymorphisms ...
Fernandez Jaén, Tomas; Álvarez Rey, Guillermo; Angulo, Francisco; Ardevol Cuesta, Jordi; Arriaza Loureda, Rafael; Ávila España, Fernando; Ayala, Juan; Balius Matas, Ramón; Baró Pazos, Fernando; Dios Beas Jiménez, Juan de; Candel Rosell, Jorge; Cobián Fernandez, César; Doñoro Cuevas, Mª del Pilar; Esparza Ros, Francisco; Espejo Colmenero, Josefina; Fernández de Prado, Jorge; García Cota, Juan José; Garrido González, Jose Ignacio; Gonzalez de Vega, Carlos; González Santander, Manuela; Herrador Munilla, Miguel Ángel; Ivorra Ruiz, Francisco; Jiménez Díaz, Fernando; Maestro Fernandez, Antonio; Manonelles Marqueta, Pedro; Muñoz Benito, Juan Jose; Olivé Vilás, Ramón; Pedret, Carles; Peirau i Terés, Xavier; Peña Amaro, José; Puigdellivoll Grifell, Jordi; Pérez San Roque, Juan; Ramírez Parenteu, Christophe; Ribas Serna, Juan; Rodas, Gil; Sánchez Álvarez, Mikel; Sanchez Marchori, Carlos; Til Perez, Lluis; Ureña Durán, Rosario; Valle Soto, Miguel de; Villalón Alonso, José María; Guillen García, Pedro; Spanish Group for Tendon Consensus (Sage, 2017)On October 15, 2016, experts met at Clínica CEMTRO in Madrid, Spain, under the patronage of the Spanish Society for Sports Traumatology (SETRADE), the Spanish Society of Sports Medicine (SEMED), the Spanish Association of ...