Universitat de Lleida
    • English
    • català
    • español
  • English 
    • English
    • català
    • español
  • Login
Repositori Obert UdL
View Item 
  •   Home
  • Recerca
  • Tecnologia dels Aliments
  • Articles publicats (Tecnologia dels Aliments)
  • View Item
  •   Home
  • Recerca
  • Tecnologia dels Aliments
  • Articles publicats (Tecnologia dels Aliments)
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Validation of dried blood spot cards to determine apple phenolic metabolites in human blood and plasma after an acute intake of red-fleshed apple snack

Thumbnail
View/Open
Postprint (1.190Mb)
Issue date
2018
Author
Yuste, Silvia
Macià i Puig, Ma Alba
Ludwig, Iziar A.
Romero Fabregat, Mª Paz
Fernández Castillejo, Sara
Catalán Santos, Úrsula
Motilva Casado, Mª José
Rubió Piqué, Laura
Suggested citation
Yuste, Silvia; Macià i Puig, Ma Alba; Ludwig, Iziar A.; Romero Fabregat, Mª Paz; Fernández Castillejo, Sara; Catalán Santos, Úrsula; ... Rubió Piqué, Laura. (2018) . Validation of dried blood spot cards to determine apple phenolic metabolites in human blood and plasma after an acute intake of red-fleshed apple snack. Molecular Nutrition & Food Research, 2016, vol. 62, núm. 23, e1800623. https://doi.org/10.1002/mnfr.201800623.
Impact


Web of Science logo    citations in Web of Science

Scopus logo    citations in Scopus

Google Scholar logo  Google Scholar
Share
Export to Mendeley
Metadata
Show full item record
Abstract
Scope: The application of dried blood spot (DBS) cards for the study in human blood of dietary polyphenol bioavailability has been poorly studied. Methods and results: An analytical method based on blood sampling with DBS cards combined with LC–MS/MS has been developed and validated. To test the method validation, the phenolic metabolites are determined in human blood and plasma obtained after an acute intake of a red-fleshed apple snack in ten volunteers. Capillary blood by finger prick is compared to venous blood by venipuncture and whole blood is also compared to their corresponding venous plasma samples. Moreover, the venous plasma results using DBS cards are compared to those obtained by microElution solid phase extraction (μSPE). The main phenolic metabolites detected in blood and plasma samples are phloretin glucuronide, dihydroxyphenylpropionic acid sulphate, (methyl) catechol sulphate, catechol glucuronide, and hydroxyphenyl-γ -valerolactone glucuronide. No significant differences are observed between capillary blood, venous blood, and plasma samples using DBS, and neither between plasma samples analyzed by DBS or μSPE. Conclusions: Finger-prick blood sampling based on DBS appears to be a suitable alternative to the classic invasive venipuncture for the determination of circulating phenolic metabolites in nutritional postprandial studies.
URI
http://hdl.handle.net/10459.1/65724
DOI
https://doi.org/10.1002/mnfr.201800623
Is part of
Molecular Nutrition & Food Research, 2016, vol. 62, núm. 23, e1800623
European research projects
Collections
  • Publicacions de projectes de recerca del Plan Nacional [2180]
  • Articles publicats (Agrotecnio Center) [931]
  • Articles publicats (Tecnologia dels Aliments) [490]

Related items

Showing items related by title, author, creator and subject.

  • In vivo biotransformation of (poly)phenols and anthocyanins of red-fleshed apple and identification of intake biomarkers 

    Yuste, Silvia; Ludwig, Iziar A.; Rubió Piqué, Laura; Romero Fabregat, Mª Paz; Pedret, Anna; Valls, Rosa M.; Solà, Rosa; Motilva Casado, Mª José; Macià i Puig, Ma Alba (Elsevier, 2019)
    The aim of this study was to investigate comprehensively the metabolic pathways and human bioavailability of anthocyanins and other phenolic compounds in apple matrix, and to elucidate potential intake biomarkers. After ...
  • Metabolite profiling of olive oil and thyme phenols after a sustained intake of two phenol-enriched olive oils by humans: Identification of compliance markers 

    Rubió Piqué, Laura; Farràs, Marta; De la Torre, Rafael; Macià i Puig, Ma Alba; Romero Fabregat, Mª Paz; Valls, Rosa M.; Solà, Rosa; Farré, Magí; Fitó, Montserrat; Motilva Casado, Mª José (Elsevier, 2014)
    An understanding of causal relations between phenol intake and its beneficial effects on health is hindered by the lack of robust biological markers of its exposure. This is particularly relevant in mid/long-term nutritional ...
  • Protective effect of hydroxytyrosol and its predominant plasmatic human metabolites against endothelial dysfunction in human aortic endothelial cells 

    Catalán Santos, Úrsula; López de las Hazas Mingo, María del Carmen; Rubió Piqué, Laura; Fernández Castillejo, Sara; Pedret, Anna; De la Torre, Rafael; Motilva Casado, Mª José; Solà, Rosa (Wiley, 2015)
    Scope Hydroxytyrosol (HT) is the major phenolic compound in virgin olive oil (VOO) in free and conjugated forms that may exert health benefits against atherosclerosis. The native form of HT is undetectable in plasma due ...

Contact Us | Send Feedback | Legal Notice
© 2021 BiD. Universitat de Lleida
Metadata subjected to 
 

 

Browse

All of the repositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

Statistics

View Usage Statistics

D'interès

Política institucional d'accés obertDiposita les teves publicacionsDiposita dades de recercaSuport a la recerca

Contact Us | Send Feedback | Legal Notice
© 2021 BiD. Universitat de Lleida
Metadata subjected to