Validation of dried blood spot cards to determine apple phenolic metabolites in human blood and plasma after an acute intake of red-fleshed apple snack

Yuste, Silvia; Macià i Puig, Ma Alba; Ludwig, Iziar A.; Romero Fabregat, Mª Paz; Fernández Castillejo, Sara; Catalán Santos, Úrsula; Motilva Casado, Mª José; Rubió Piqué, Laura

doi:https://doi.org/10.1002/mnfr.201800623

View/Open

Postprint (1.190Mb)

Issue date

2018

Author

Yuste, Silvia

Macià i Puig, Ma Alba

Ludwig, Iziar A.

Romero Fabregat, Mª Paz

Fernández Castillejo, Sara

Catalán Santos, Úrsula

Motilva Casado, Mª José

Rubió Piqué, Laura

Suggested citation

Yuste, Silvia; Macià i Puig, Ma Alba; Ludwig, Iziar A.; Romero Fabregat, Mª Paz; Fernández Castillejo, Sara; Catalán Santos, Úrsula; ... Rubió Piqué, Laura. (2018) . Validation of dried blood spot cards to determine apple phenolic metabolites in human blood and plasma after an acute intake of red-fleshed apple snack. Molecular Nutrition & Food Research, 2016, vol. 62, núm. 23, e1800623. https://doi.org/10.1002/mnfr.201800623.

Abstract

Scope: The application of dried blood spot (DBS) cards for the study in human blood of dietary polyphenol bioavailability has been poorly studied. Methods and results: An analytical method based on blood sampling with DBS cards combined with LC–MS/MS has been developed and validated. To test the method validation, the phenolic metabolites are determined in human blood and plasma obtained after an acute intake of a red-fleshed apple snack in ten volunteers. Capillary blood by finger prick is compared to venous blood by venipuncture and whole blood is also compared to their corresponding venous plasma samples. Moreover, the venous plasma results using DBS cards are compared to those obtained by microElution solid phase extraction (μSPE). The main phenolic metabolites detected in blood and plasma samples are phloretin glucuronide, dihydroxyphenylpropionic acid sulphate, (methyl) catechol sulphate, catechol glucuronide, and hydroxyphenyl-γ -valerolactone glucuronide. No significant differences are observed between capillary blood, venous blood, and plasma samples using DBS, and neither between plasma samples analyzed by DBS or μSPE. Conclusions: Finger-prick blood sampling based on DBS appears to be a suitable alternative to the classic invasive venipuncture for the determination of circulating phenolic metabolites in nutritional postprandial studies.

URI

http://hdl.handle.net/10459.1/65724

DOI

https://doi.org/10.1002/mnfr.201800623

Is part of

Molecular Nutrition & Food Research, 2016, vol. 62, núm. 23, e1800623