A Thioredoxin Domain-Containing Protein Interacts with Pepino mosaic virus Triple Gene Block Protein 1

View/ Open
Issue date
2018Author
Mathioudakis, Matthaios M.
Khechmar, Souheyla
Owen, Carolyn A.
Mansour, Karima Ben
Tomaszewska, Weronika
Spanos, Theodore
Sarris, Panagiotis F.
Livieratos, Ioannis C.
Suggested citation
Mathioudakis, Matthaios M.;
Khechmar, Souheyla;
Owen, Carolyn A.;
Medina Piles, Vicente;
Mansour, Karima Ben;
Tomaszewska, Weronika;
...
Livieratos, Ioannis C..
(2018)
.
A Thioredoxin Domain-Containing Protein Interacts with Pepino mosaic virus Triple Gene Block Protein 1.
International Journal of Molecular Sciences, 2018, vol. 19, núm. 12, 3747.
https://doi.org/10.3390/ijms19123747.
Metadata
Show full item recordAbstract
Pepino mosaic virus (PepMV) is a mechanically-transmitted tomato pathogen of importance worldwide. Interactions between the PepMV coat protein and triple gene block protein (TGBp1) with the host heat shock cognate protein 70 and catalase 1 (CAT1), respectively, have been previously reported by our lab. In this study, a novel tomato interactor (SlTXND9) was shown to bind the PepMV TGBp1 in yeast-two-hybrid screening, in vitro pull-down and bimolecular fluorescent complementation (BiFC) assays. SlTXND9 possesses part of the conserved thioredoxin (TRX) active site sequence (W__PC vs. WCXPC), and TXND9 orthologues cluster within the TRX phylogenetic superfamilyclosesttophosducin-likeprotein-3. InPepMV-infectedandhealthyNicotianabenthamiana plants,NbTXND9mRNAlevelswerecomparable,andexpressionlevelsremainedstableinbothlocal and systemic leaves for 10 days post inoculation (dpi), as was also the case for catalase 1 (CAT1). To localize the TXND9 in plant cells, a polyclonal antiserum was produced. Purified α-SlTXND9 immunoglobulin (IgG) consistently detected a set of three protein bands in the range of 27–35 kDa, in the 1000 and 30,000 g pellets, and the soluble fraction of extracts of healthy and PepMV-infected N. benthamiana leaves, but not in the cell wall. These bands likely consist of the homologous protein NbTXND9 and its post-translationally modified derivatives. On electron microscopy, immuno-gold labellingofultrathinsectionsofPepMV-infectedN.benthamianaleavesusingα-SlTXND9IgGrevealed particle accumulation close to plasmodesmata, suggesting a role in virus movement. Taken together, this study highlights a novel tomato-PepMV protein interaction and provides data on its localization in planta. Currently, studies focusing on the biological function of this interaction during PepMV infection are in progress.
Is part of
International Journal of Molecular Sciences, 2018, vol. 19, núm. 12, 3747European research projects
Collections
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as cc-by (c) Matthaios M. Mathioudakis et al., 2018
Related items
Showing items related by title, author, creator and subject.
-
Crinivirus replication and host interactions
Kiss, Zsofia Agnes; Medina Piles, Vicente; Falk, Bryce W. (Frontiers, 2013)Criniviruses comprise one of the genera within the family Closteroviridae. Members in this family are restricted to the phloem and rely on whitefly vectors of the genera Bemisia and/or Trialeurodes for plant-to-plant ... -
A distinct, non-virion plant virus movement protein encoded by a crinivirus essential for systemic infection
Qiao, Wenjie; Medina Piles, Vicente; Kuo, Yen-Wen; Falk, Bryce W. (American Society for Microbiology, 2018)Plant-infecting viruses utilize various strategies involving multiple viral and host factors to achieve successful systemic infections of their compatible hosts. Lettuce infectious yellows virus (LIYV), genus Crinivirus, ... -
RNA2‐encoded VP37 protein of Broad bean wilt virus 1 is a determinant of pathogenicity, host susceptibility, and a suppressor of post‐transcriptional gene silencing
Carpino, Caterina; Ferriol Safont, Inmaculada; Elvira-González, Laura; Medina Piles, Vicente; Rubio, Luis; Peri, Ezio; Davino, Salvatore; Galipienso Torregrosa, Luis (British Society for Plant PathologyJohn Wiley & Sons Ltd, 2020-09-16)Broad bean wilt virus 1 (BBWV‐1, genus Fabavirus, family Secoviridae) is a bipartite, single‐stranded positive‐sense RNA virus infecting many horticultural and ornamental crops worldwide. RNA1 encodes proteins involved in ...