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dc.contributor.authorRoyo Sánchez-Palencia, José Luis
dc.contributor.authorHidalgo, Carmen
dc.contributor.authorRoncero, Yolanda
dc.contributor.authorSeda, María Ángeles
dc.contributor.authorAkalin, Altuna
dc.contributor.authorLenhard, Boris
dc.contributor.authorCasares, Fernando
dc.contributor.authorGómez-Skarmeta, José Luis
dc.description.abstractNon-coding DNA conservation across species has been often used as a predictor for transcriptional enhancer activity. However, only a few systematic analyses of the function of these highly conserved non-coding regions (HCNRs) have been performed. Here we use zebrafish transgenic assays to perform a systematic study of 113 HCNRs from human chromosome 16. By comparing transient and stable transgenesis, we show that the first method is highly inefficient, leading to 40% of false positives and 20% of false negatives. When analyzed in stable transgenic lines, a great majority of HCNRs were active in the central nervous system, although some of them drove expression in other organs such as the eye and the excretory system. Finally, by testing a fraction of the HCNRs lacking enhancer activity for in vivo insulator activity, we find that 20% of them may contain enhancer-blocking function. Altogether our data indicate that HCNRs may contain different types of cisregulatory activity, including enhancer, insulators as well as other not yet discovered functions.ca_ES
dc.description.sponsorshipThis work was funded by the Spanish and Andalusian Governments, grants BFU2010-14839, BFU2009-07044, CSD2007-00008, Proyecto de Excelencia CVI-3488 and CVI-2658.ca_ES
dc.publisherPublic Library of Scienceca_ES
dc.relation.isformatofReproducció del document publicat a
dc.relation.ispartofPLoS ONE, 2011, vol. 6, núm 9, e24824ca_ES
dc.rightscc-by (c) José Luis Royo et al., 2011ca_ES
dc.titleDissecting the Transcriptional Regulatory Properties of Human Chromosome 16 Highly Conserved Non-Coding Regionsca_ES

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cc-by (c) José Luis Royo et al., 2011
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