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dc.contributor.authorVilanova Torren, Laura
dc.contributor.authorLópez-Pérez, M.
dc.contributor.authorBallester, Ana-Rosa
dc.contributor.authorTeixidó i Espasa, Neus
dc.contributor.authorUsall i Rodié, Josep
dc.contributor.authorLara Ayala, Isabel
dc.contributor.authorViñas Almenar, Inmaculada
dc.contributor.authorTorres Sanchis, Rosario
dc.contributor.authorGonzález-Candelas, Luis
dc.description.abstractThe fungus Penicillium digitatum is the causal agent of the citrus green mould, the major postharvest diseases of citrus fruit. Lesions on the surface of infected fruits first appear as soft areas around the inoculation site, due to maceration of fruit. The macerating activity has been associated with pectinases secreted by the fungus during infection. In order to evaluate the contribution to virulence and macerating activity of the two major polygalacturonases (PGs) secreted by P. digitatum, we have obtained and characterized mutants lacking either pg1 or pg2, the genes encoding PG1 and PG2, respectively. Disease incidence of deletants in either gene was not different from that of the parental strain or ectopic transformants. However, disease progressed more slowly in deletants, especially in those lacking the pg2 gene. The lesions originated by the Δpg2 deletants were not as soft and the pH was not as acid as those originated by either the wild type strain or the ectopic transformants. Total PG activity in the macerated tissue was also lower in fruits infected with the Δpg2 deletants. Interestingly, the macerated tissue of oranges infected with Δpg2 deletants showed around 50% reduction in galacturonic acid content with respect to lesions caused by any other strain.ca_ES
dc.description.sponsorshipThe technical assistance of Ana Izquierdo is gratefully acknowledged. LG-C's research is funded in part by the Spanish Ministry of Economy, Industry and Competitiveness (AGL2011-30519-C03-01 and AGL2014-55802-R) and the Generalitat Valenciana (PROMETEOII/2014/027). ML-P was supported by a “Formación de Personal Investigador” scholarship (BES-2006-12983). Authors want to thank the technical assistance of S. Dashevskaya and the financial support by AGL2011-30519-CO3-03 from the “Ministerio de Economía y Competitividad” (MINECO, Spain), and the CERCA Programme/Generalitat de Catalunya.ca_ES
dc.relation.isformatofVersió postprint del document publicat a:
dc.relation.ispartofInternational Journal of Food Microbiology, 2018, vol. 282, p. 16-23ca_ES
dc.rightscc-by-nc-nd (c) Elsevier, 2018ca_ES
dc.subjectPostharvest pathologyca_ES
dc.subjectGreen mouldca_ES
dc.subjectGalacturonic acidca_ES
dc.titleDifferential contribution of the two major polygalacturonases from Penicillium digitatum to virulence towards citrus fruitca_ES

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cc-by-nc-nd (c) Elsevier, 2018
Except where otherwise noted, this item's license is described as cc-by-nc-nd (c) Elsevier, 2018