Adipose TSHB in Humans and Serum TSH in Hypothyroid Rats Inform About Cellular Senescence

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2018-11-18
Author
Moreno-Navarrete, José María
Liñares-Pose, Laura
Sabater, Mónica
Rial-Pensado, Eva
Comas, Ferran
Jové Font, Mariona
Latorre, Jèsica
Ortega Delgado, Francisco José
Ricart, Wifredo
Portero Otín, Manuel
López, Miguel
Fernández Real, José Manuel
Suggested citation
Moreno-Navarrete, José María; Liñares-Pose, Laura; Sabater, Mónica; Rial-Pensado, Eva; Comas, Ferran; Jové Font, Mariona; ... Fernández Real, José Manuel. (2018) . Adipose TSHB in Humans and Serum TSH in Hypothyroid Rats Inform About Cellular Senescence. Cellular Physiology and Biochemistry, 2018, vol. 51, núm. 1, p. 142-153. https://doi.org/10.1159/000495170.
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Abstract
Background/Aims: Thyroid hormones have been recently linked to senescence and longevity. Given the recent description of TSHB mRNA in human adipose tissue (AT), we aimed to investigate the relationship between local AT TSH and adipose tissue senescence. Methods: TSHB mRNA (measured by real-time PCR) and markers of adipose tissue senescence [BAX, DBC1, TP53, TNF (real-time PCR), telomere length (Telo TAGGG Telomere Length Assay) and lipidomics (liquid chromatography mass spectrometry)] were analysed in subcutaneous (SAT) and visceral (VAT) AT from euthyroid subjects. The chronic effects of TSH were also investigated in AT from hypothyroid rats and after recombinant human TSH (rhTSH) administration in human adipocytes. Results: Both VAT and SAT TSHB gene expression negatively correlated with markers of AT cellular senescence (BAX, DBC1, TP53, TNF gene expression and specific glucosylceramides) and positively associated with telomere length. Supporting these observations, both rhTSH administration in human adipocytes and increased TSH in hypothyroid rats resulted in decreased markers of cellular senescence (Bax and Tp53 mRNA) in both gonadal and subcutaneous white adipose tissue. Conclusion: These data point to a possible role of TSH in AT cellular senescence.
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http://hdl.handle.net/10459.1/65270
DOI
https://doi.org/10.1159/000495170
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Cellular Physiology and Biochemistry, 2018, vol. 51, núm. 1, p. 142-153
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cc-by-nc-nd (c) José María Moreno-Navarrete et al., 2018
Except where otherwise noted, this item's license is described as cc-by-nc-nd (c) José María Moreno-Navarrete et al., 2018