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dc.contributor.authorSolórzano, Alicia
dc.contributor.authorFoni, Emanuela
dc.contributor.authorCórdoba, Lorena
dc.contributor.authorBaratelli, Massimiliano
dc.contributor.authorRazzuoli, Elisabetta
dc.contributor.authorBilato, Dania
dc.contributor.authorMartín del Burgo, María Ángeles
dc.contributor.authorPerlin, David S.
dc.contributor.authorMartínez, Jorge
dc.contributor.authorMartínez-Orellana, Pamela
dc.contributor.authorFraile Sauce, Lorenzo José
dc.contributor.authorChiapponi, Chiara
dc.contributor.authorAmadori, Massimo
dc.contributor.authorDel Real, Gustavo
dc.contributor.authorMontoya, Maria
dc.date.accessioned2018-11-28T10:49:10Z
dc.date.available2018-11-28T10:49:10Z
dc.date.issued2015
dc.identifier.issn0022-538X
dc.identifier.urihttp://hdl.handle.net/10459.1/65208
dc.description.abstractAvian influenza A viruses have gained increasing attention due to their ability to cross the species barrier and cause severe disease in humans and other mammal species as pigs. H3 and particularly H3N8 viruses, are highly adaptive since they are found in multiple avian and mammal hosts. H3N8 viruses have not been isolated yet from humans; however, a recent report showed that equine influenza A viruses (IAVs) can be isolated from pigs, although an established infection has not been observed thus far in this host. To gain insight into the possibility of H3N8 avian IAVs to cross the species barrier into pigs, in vitro experiments and an experimental infection in pigs with four H3N8 viruses from different origins (equine, canine, avian, and seal) were performed. As a positive control, an H3N2 swine influenza virus A was used. Although equine and canine viruses hardly replicated in the respiratory systems of pigs, avian and seal viruses replicated substantially and caused detectable lesions in inoculated pigs without previous adaptation. Interestingly, antibodies against hemagglutinin could not be detected after infection by hemagglutination inhibition (HAI) test with avian and seal viruses. This phenomenon was observed not only in pigs but also in mice immunized with the same virus strains. Our data indicated that H3N8 IAVs from wild aquatic birds have the potential to cross the species barrier and establish successful infections in pigs that might spread unnoticed using the HAI test as diagnostic tool.
dc.description.sponsorshipWe thank Jaime Maldonado and HIPRA (Spain) for the A/Swine/Spain/ 54008/2004 (H3N2) strain, Edward J. Dubovi and Cornell University for the A/Canine/NY/105447/08 (H3N8) IAV strain, T. M. Chambers and the University of Kentucky for the A/Equine/OH/1/03 (H3N8) IAV strain, and Hon Ip and the U.S. Geological Survey National Wildlife Health Center for the A/American black duck/Maine/44411-532/2008 (H3N8) and the A/Harbor Seal/New Hampshire/179629/2011 (H3N8) IAV strains. We thank Sergio López, David Solanes, Francisco X. Abad, Jordi Alberola, Jaume Martorell, and Eduard J. Cunilleras for help in providing different samples and during the experimental infections, as well as the personnel in Cat3 laboratories and the animal house. We thank Adolfo García-Sastre for providing materials and for support as the principal investigator of the NIAID-funded Center for Research in Influenza Pathogenesis (HHSN266200700010C). The research leading to these results received funding from the European Community’s Seventh Framework Programme (FP7, 2007-2013), the Research Infrastructures Action under grant FP7-228393 (a NADIR project), and projects AGL2010-22200-C02-01 and AGL2007-60274 of the Spanish Ministry of Science and Innovation.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherAmerican Society for Microbiology
dc.relationMICINN/PN2008-2011/AGL2010-22200-C02-01
dc.relationMIECI/PN2004-2007/AGL2007-60274
dc.relation.isformatofVersió postprint del document publicat a: https://doi.org/10.1128/JVI.01509-15
dc.relation.ispartofJournal of Virology, 2015, vol. 89, núm. 22, p. 11190-11202
dc.rights(c) American Society for Microbiology, 2015
dc.subject.otherPorcs--Infeccions
dc.subject.otherAnimals--Malalties
dc.subject.otherPorcs--Virus
dc.titleCross-species infectivity of H3N8 influenza virus in an experimental infection in swine
dc.typeinfo:eu-repo/semantics/article
dc.date.updated2018-11-28T10:49:10Z
dc.identifier.idgrec023123
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.identifier.doihttps://doi.org/10.1128/JVI.01509-15
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/228393


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