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dc.contributor.authorLao, Mireia
dc.contributor.authorDago Busquets, Àngela
dc.contributor.authorSerrano, N.
dc.contributor.authorCompanys Ferran, Encarnació
dc.contributor.authorPuy Llorens, Jaume
dc.contributor.authorGalceran i Nogués, Josep
dc.date.accessioned2018-11-22T08:41:42Z
dc.date.available2018-11-22T08:41:42Z
dc.date.issued2015-11-01
dc.identifier.issn1572-6657
dc.identifier.urihttp://hdl.handle.net/10459.1/65162
dc.description.abstractZn-Glutathione speciation was studied applying the electrochemical technique AGNES (Absence of Gradients and Nernstian Equilibrium Stripping) to determine the free zinc concentration. In titrations varying either pH, total concentration of gluthatione (cT,GSH) or total concentration of Zn (cT,Zn), free Zn concentrations determined with AGNES were compared with the values predicted from previously reported complexation constants. The speciation of Zn was studied in a real sample of root extracts of Hordeum vulgare where the cT,Zn had been determined by ICP-MS and cT,GSH by HPLC. The free [Zn2+] was measured with AGNES using a special device where a mixture of N2/CO2 saturated in milliQ water controls the pH and avoids the evaporation of the sample. The lower free zinc concentration determined with AGNES, in comparison with the predicted one assuming the literature complexation constants and taking into account only the presence of Zn and GSH, indicates that it is necessary to include more ligands apart from GSH (as other phytochelatins) in the speciation model.
dc.description.sponsorshipThe authors gratefully acknowledge support of this research by the Spanish Ministry Spanish Ministry of Education and Science (Projects CTQ2009-07831, CTM2012-39183, CTQ2012-32863 and CTM2013-48967) and by the "Comissionat d'Universitats i Recerca de la Generalitat de Catalunya” (2014SGR269 and 2014SGR1132). ML acknowledges a FPI grant from the Spanish Ministry.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherElsevier
dc.relationMCINN/PN2008-2011/CTQ2009-07831
dc.relationMICINN/PN2008-2011/CTM2012-39183
dc.relationMICINN/PN2008-2011/ CTQ2012-32863
dc.relationMINECO/PN2013-2016/CTM2013-48967
dc.relation.isformatofVersió postprint del document publicat a: https://doi.org/10.1016/j.jelechem.2015.08.029
dc.relation.ispartofJournal of Electroanalytical Chemistry, 2015, vol. 756, p. 207-211
dc.rightscc-by-nc-nd (c) Elsevier, 2015
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAbsence of Gradients and Nernstian Equilibrium Stripping
dc.subjectSpeciation techniques
dc.subjectZinc complexes
dc.subjectEnvironmental analysis
dc.titleFree Zn2+ determination in systems with Zn-Glutathione
dc.typeinfo:eu-repo/semantics/article
dc.date.updated2018-11-22T08:41:43Z
dc.identifier.idgrec023379
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.identifier.doihttps://doi.org/10.1016/j.jelechem.2015.08.029


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cc-by-nc-nd (c) Elsevier, 2015
Except where otherwise noted, this item's license is described as cc-by-nc-nd (c) Elsevier, 2015