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dc.contributor.authorIgartua Arregui, Ernesto
dc.contributor.authorMansour Elsayed, Elsayed
dc.contributor.authorCantalapiedra, Carlos P.
dc.contributor.authorContreras‐Moreira, Bruno
dc.contributor.authorGracia Gimeno, Ma. Pilar
dc.contributor.authorFuster, Prudencio
dc.contributor.authorEscribano, J.
dc.contributor.authorMolina Cano, José Luis
dc.contributor.authorMoralejo Vidal, Mª Angeles
dc.contributor.authorCiudad, Francisco J.
dc.contributor.authorThomas, William T. B.
dc.contributor.authorKarsai, Ildikó
dc.contributor.authorCasas Cendoya, Ana Maria
dc.date.accessioned2018-11-08T10:21:40Z
dc.date.available2018-11-08T10:21:40Z
dc.date.issued2015
dc.identifier.issn1380-3743
dc.identifier.urihttp://hdl.handle.net/10459.1/65053
dc.description.abstractThis study is a retrospective analysis of an elite cross from the Spanish National Barley Breeding Program. This was the most successful cross produced in the breeding program in the past 20 years. The progeny from this cross has been investigated at two points in the program, before and after selection, through the analysis of allelic frequencies at a number of genetic loci with molecular markers. Shifts in allelic frequencies after selection allowed the identification of genomic regions with selection footprints likely due to the breeding process. The cross was replicated in three different years, and therefore, the three progenies represent different selection histories but, in all cases, were preferentially selected compared to the lines from other crosses used in the program. The progenies were sampled at two generations, before conscious selection (F2) and after six generations of selection (F8). The F2 plants were genotyped with microsatellites, whereas 31 F8 lines were surveyed for SNP and presence/absence variation polymorphisms using a genotyping-by-sequencing system (DArTseq). The DArTseq markers were aligned to the barley physical map, and, after curation, over 3,000 were still available for the analysis. Overall, 15 genomic regions in the F8 lines had allele frequencies beyond chosen thresholds, indicating selection, eight toward parent Orria and seven toward Plaisant. These selection footprints partially validated QTLs detected through classical linkage mapping in a RIL population of the same cross. These validated selection footprints convey useful information for barley breeding, either through marker-assisted selection or through genomic selection.ca_ES
dc.description.sponsorshipThis work was supported by the Spanish Ministry of Science and Innovation (MICINN), who funded this work with the scholarship BES-2008-009623 (EM), and the projects AGL2010-21929, RTA03-028-C4, RTA2006-00020-C04 and RTA2009-00006-C04, and by Grant A06 from the Aragon Government. Genotype by sequencing was funded by DGA—Obra Social La Caixa (Grant Number GA-LC-059-2011). WTBT acknowledges funding by the Scottish Government’s Rural and Environment Science and Analytical Services Division.ca_ES
dc.language.isoengca_ES
dc.publisherSpringerca_ES
dc.relationMICINN/PN2008-2011/AGL2010-21929
dc.relationMICYT/PN2000-2003/RTA2003-028-C4
dc.relationMIECI/PN2004-2007/RTA2006-00020-C04
dc.relationMICINN/PN2008-2011/RTA2009-00006-C04
dc.relation.isformatofVersió postprint del document publicat a https://doi.org/10.1007/s11032-015-0194-8ca_ES
dc.relation.ispartofMolecular Breeding, 2015, vol. 35, núm. 11ca_ES
dc.rights(c) Springer, 2015ca_ES
dc.subjectBarleyca_ES
dc.subjectBreedingca_ES
dc.subjectSelection footprintca_ES
dc.subjectBarley Breeding Selection footprint Genotyping-by-sequencingca_ES
dc.titleSelection footprints in barley breeding lines detected by combining genotyping-by-sequencing with reference genome informationca_ES
dc.typeinfo:eu-repo/semantics/articleca_ES
dc.identifier.idgrec021001
dc.type.versioninfo:eu-repo/semantics/acceptedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.identifier.doihttps://doi.org/10.1007/s11032-015-0194-8


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