Studies on the biocontrol mechanisms of Pseudomonas graminis strain CPA-7 against food-borne pathogens in vitro and on fresh-cut melon

Collazo Cordero, Cyrelys; Abadias i Sero, Mª Isabel; Aguiló-Aguayo, Ingrid; Alegre Vilas, Isabel; Chenoll, Empar; Viñas Almenar, Inmaculada

doi:https://doi.org/10.1016/j.lwt.2017.02.029

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2017

Author

Collazo Cordero, Cyrelys

Abadias i Sero, Mª Isabel

Aguiló-Aguayo, Ingrid

Alegre Vilas, Isabel

Chenoll, Empar

Viñas Almenar, Inmaculada

Suggested citation

Collazo Cordero, Cyrelys; Abadias i Sero, Mª Isabel; Aguiló-Aguayo, Ingrid; Alegre Vilas, Isabel; Chenoll, Empar; Viñas Almenar, Inmaculada; . (2017) . Studies on the biocontrol mechanisms of Pseudomonas graminis strain CPA-7 against food-borne pathogens in vitro and on fresh-cut melon. LWT - Food Science and Technology, 2017, vol. 85, Part B, p. 301-308. https://doi.org/10.1016/j.lwt.2017.02.029.

Abstract

The present study was aimed at gaining insight into the mode of action of the antagonistic bacteria Pseudomonas graminis CPA-7, which has been previously identified as an effective biocontrol agent against Listeria monocytogenes, Salmonella enterica and Escherichia coli O157:H7 on fresh-cut fruit. In

vitro experiments did not reveal any antimicrobial or proteolytic activity on solid media or any biosurfactant activity on hydrophobic surfaces. Metabolites produced by CPA-7 in two different culture media and on 'Galia' melon were unable to inhibit L. monocytogenes populations on 'Galia' melon plugs at 25 °C or 5 °C. In contrast, at 25 °C the population of this pathogen on 'Galia' plugs was reduced by 2.1 and 3.3 log-units when co-inoculated with the antagonist in water, after 24 and 48 h, respectively. CPA-7 did not form biofilms after 72 h at 25 °C (OD = 0.03) or at 30 °C (OD = 0.01) on polystyrene plates and the production of alginate was close to the negative control. Studies of nutritional profiles showed high overlap (NOI > 0.9) between CPA-7 and E. coli O157:H7 regarding the use of carboxylic acids. This functional group could also contain putative targets for competiveness between CPA-7 and S. enterica, although overlapping was not restrictive enough (NOI = 0.83).

URI

http://hdl.handle.net/10459.1/65019

DOI

https://doi.org/10.1016/j.lwt.2017.02.029

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LWT - Food Science and Technology, 2017, vol. 85, Part B, p. 301-308

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Except where otherwise noted, this item's license is described as cc-by-nc-nd (c) Elsevier, 2017