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dc.contributor.authorTarradas, Joan
dc.contributor.authorDe la Torre Martínez, Ma. Eugènia
dc.contributor.authorRosell, Rosa
dc.contributor.authorPerez, Lester Josue
dc.contributor.authorPujols, Joan
dc.contributor.authorMuñoz, Marta
dc.contributor.authorMuñoz, Iván
dc.contributor.authorMuñoz, Sara
dc.contributor.authorAbad, Xavier
dc.contributor.authorDomingo, Mariano
dc.contributor.authorFraile Sauce, Lorenzo José
dc.contributor.authorGanges, Llilianne
dc.description.abstractThe severity of the acute form of CSF is responsible for the high mortality rate and has been the subject of many studies. Nevertheless, some animals are likely to develop a mild, chronic, or unapparent form of the disease. Paradoxically, this clinical form of the disease has not been well studied, especially regarding its pathogenesis. In this study, we investigated the infection in domestic pigs that is caused by the CSFV Cat01 strain, which is responsible for the 2001-2002 CSFV outbreak in Catalonia, Spain, and which caused mild and nonspecific clinical signs compared to the infection that is caused by another CSFV strain that is responsible for inducing severe clinical symptoms of disease. We assessed the impact of the CSFV infection in the immune system of domestic pigs, mainly on the kinetics of different cytokines, such as IFN-α (innate immunity) and IFN-γ (adaptive immune response), during the first weeks after infection. In addition, we evaluated the impact on the induction of the humoral response and its relation to the course of infection and the RNA CSFV viral load. The IFN-α levels in the serum samples from the pigs that developed a milder form of the CSF disease (infected with Cat01 strain) were lower than those that were detected in the pig with severe clinical CSF signs (Margarita strain). After infection with Cat01 strain, the IFN-γ levels in response to CSFV were detected in addition to the humoral response. Interestingly, in the serum samples of these animals, we detected the lowest load of CSFV RNA. Similarly, the lowest viral load levels were detected in the tonsils of these pigs. Both the T cells and the humoral response that were generated in most of the pigs that were infected with strain Cat01 may be related to the protection in the symptom progression of CSF against this viral strain. These results explain the antiviral role of IFN-γ in the absence of an antibody response. Likewise, these results corroborate the relevance and relationship that exists between the intensity of the T cell response and the protection against CSFV replication. Additionally, these results also explain how the failure to induce optimal levels of humoral and cellular responses after CSFV infection promotes the spread and persistence of the virus.
dc.description.sponsorshipWe thank Iván Cordón and David Solanes for their help in theanimal facilities. This research was supported by grant AGL2012-38343 from the Spanish Government.
dc.relation.isformatofVersió postprint del document publicat a:
dc.relation.ispartofVirus Research, 2014, vol. 185, p. 82-91
dc.rightscc-by-nc-nd (c) Elsevier, 2014
dc.subjectVirus detection
dc.subjectImmune response
dc.subjectCourse of infection
dc.titleThe impact of CSFV on the immune response to control infection

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cc-by-nc-nd (c) Elsevier, 2014
Except where otherwise noted, this item's license is described as cc-by-nc-nd (c) Elsevier, 2014