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dc.contributor.authorBelenguer, Álvaro
dc.contributor.authorBalcells Terés, Joaquim
dc.contributor.authorGuada, J. A.
dc.contributor.authorDecoux, Marc
dc.contributor.authorMilne, Eric
dc.date.accessioned2018-10-17T08:10:52Z
dc.date.available2018-10-17T08:10:52Z
dc.date.issued2005
dc.identifier.issn0007-1145
dc.identifier.urihttp://hdl.handle.net/10459.1/64897
dc.description.abstractTo study the absorption of microbial lysine in growing rabbits, a labelled diet (supplemented with 15NH4Cl) was administered to six animals (group ISOT); a control group (CTRL, four rabbits) received a similar, but unlabelled, diet. Diets were administered for 30 d. An additional group of six animals were fed the unlabelled diet for 20 d and then the labelled diet for 10 d while wearing a neck collar to avoid caecotrophy (group COLL), in order to discriminate it from direct intestinal absorption. At day 30 animals were slaughtered and caecal bacteria and liver samples taken. The 15N enrichment in amino acids of caecal bacteria and liver were determined by GC–combustion/isotope ratio MS. Lysine showed a higher enrichment in caecal microflora (0·925 atom% excess, APE) than liver (0·215 APE) in group ISOT animals, confirming the double origin of body lysine: microbial and dietary. The COLL group showed a much lower enrichment in tissue lysine (0·007 (SE 0·0029) APE for liver). Any enrichment in the latter animals was due to direct absorption of microbial lysine along the digestive tract, since recycling of microbial protein (caecotrophy) was avoided. In such conditions liver enrichment was low, indicating a small direct intestinal absorption. From the ratio of [15N]lysine enrichment between liver and bacteria the contribution of microbes to body lysine was estimated at 23 %, with 97% of this arising through caecotrophy. Absorption of microbial lysine through caecotrophy was 119 (SE 4·0) mg/d, compared with 406 (SE 1·8) mg/d available from the diet. This study confirms the importance of caecotrophy in rabbit nutrition (15% of total protein intake).ca_ES
dc.description.sponsorshipThe work was financed by Agribrands Europe España SA, Barcelona, Spain and the Spanish Ministry of Science through Projects PTR 95/0405-OP and PB98-01 601. A. B. was supported by a fellowship from the Spanish Science and Technology Ministryca_ES
dc.language.isoengca_ES
dc.publisherCambridge University Pressca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1079/BJN20051508ca_ES
dc.relation.ispartofBritish Journal of Nutrition, 2005, vol. 94, p. 763-770ca_ES
dc.rights(c) Cambridge University Press, 2005ca_ES
dc.subjectRabbitca_ES
dc.subjectCaecotrophyca_ES
dc.subjectMicrobial lysineca_ES
dc.subject15N kineticsca_ES
dc.titleProtein recycling in growing rabbits: contribution of microbial lysine to amino acid metabolismca_ES
dc.typeinfo:eu-repo/semantics/articleca_ES
dc.identifier.idgrec023014
dc.type.versioninfo:eu-repo/semantics/publishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.identifier.doihttps://doi.org/10.1079/BJN20051508


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