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dc.contributor.authorGuerouali, Abdelhai
dc.contributor.authorEl Gass, Youssef
dc.contributor.authorBalcells Terés, Joaquim
dc.contributor.authorBelenguer, Álvaro
dc.contributor.authorNolan, John
dc.date.accessioned2018-10-17T07:59:06Z
dc.date.available2018-10-17T07:59:06Z
dc.date.issued2004
dc.identifier.issn0007-1145
dc.identifier.urihttp://hdl.handle.net/10459.1/64895
dc.description.abstractFive experiments were carried out to extend knowledge of purine metabolism in the camel (Camelus dromedarius) and to establish a model to enable microbial protein outflow from the forestomachs to be estimated from the urinary excretion of purine derivatives (PD; i.e. xanthine, hypoxanthine, uric acid, allantoin). In experiment 1, four camels were fasted for five consecutive days to enable endogenous PD excretion in urine to be determined. Total PD excretion decreased during the fasting period to 267 (SE 41·5)mmol/kg body weight (W)0·75 per d. Allantoin and xanthine þ hypoxanthine were consistently 86 and 6·1% of total urinary PD during this period but uric acid increased from 3·6% to 7·4%. Xanthine oxidase activity in tissues (experiment 2) was (mmol/min per g fresh tissue) 0·038 in liver and 0·005 in gut mucosa but was not detected in plasma. In experiment 3, the duodenal supply of yeast containing exogenous purines produced a linear increase in urinary PD excretion rate with the slope indicating that 0·63 was excreted in urine. After taking account of endogenous PD excretion, the relationship can be used to predict purine outflow from the rumen. From the latter prediction, and also the purine:protein ratio in bacteria determined in experiment 5, we predicted the net microbial outflow from the rumen. In experiment 4, with increasing food intake, the rate of PD excretion in the urine increased linearly by about 11·1 mmol PD/kg digestible organic matter intake (DOMI), equivalent to 95 g microbial protein/kg DOMI.ca_ES
dc.description.sponsorshipThe present study was supported by ACSAD and the IAEA via research contract MOR10564 (Morocco) and AA.EE/ AECI 229-188 and CICYT- PB 98-1601 (Spain).ca_ES
dc.language.isoengca_ES
dc.publisherCambridge University Pressca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1079/BJN20041208ca_ES
dc.relation.ispartofBritish Journal of Nutrition, 2004, vol. 792, p. 225-232ca_ES
dc.rights(c) Cambridge University Press, 2004ca_ES
dc.subjectPurine derivativesca_ES
dc.subjectXanthine oxidaseca_ES
dc.subjectMicrobial proteinca_ES
dc.subjectCamelsca_ES
dc.titleUrinary excretion of purine derivatives as an index of microbial protein synthesis in the camel (Camelus dromedarius)ca_ES
dc.typeinfo:eu-repo/semantics/articleca_ES
dc.identifier.idgrec023007
dc.type.versioninfo:eu-repo/semantics/publishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.identifier.doihttps://doi.org/10.1079/BJN20041208


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