Epicatechin induces NF-kB, activator protein-1 (AP-1) and nuclear transcription factor erythroid 2p45-related factor-2 (Nrf2) via phosphatidylinositol-3-kinase/protein kinase B (PI3K/AKT) and extracellular regulated kinase (ERK) signalling in HepG2 cells
Martín, María Angeles
MetadataShow full item record
The dietary flavonoid epicatechin has been reported to exhibit a wide range of biological activities. The objective of the present study was to investigate the time-dependent regulation by epicatechin on the activity of the main transcription factors (NF-kB, activator protein-1 (AP-1) and nuclear transcription factor erythroid 2p45-related factor (Nrf2)) related to antioxidant defence and survival and proliferation pathways in HepG2 cells. Treatment of cells with 10mM-epicatechin induced the NF-kB pathway in a time-dependent manner characterised by increased levels of IkB kinase (IKK) and phosphorylated inhibitor of kB subunit-a (p-IkBa) and proteolytic degradation of IkB, which was consistent with an up-regulation of the NF-kB-binding activity. Time-dependent activation of the AP-1 pathway, in concert with enhanced c-Jun nuclear levels and induction of Nrf2 translocation and phosphorylation were also demonstrated. Additionally, epicatechin-induced NF-kB and Nrf2 were connected to reactive oxygen species intracellular levels and to the activation of cell survival and proliferation pathways, being phosphatidylinositol-3-kinase/protein kinase B (PI3K/AKT) and extracellular regulated kinase (ERK) associated to Nrf2 modulation and ERK to NF-kB induction. These data suggest that the epicatechin-induced survival effect occurs by the induction of redox-sensitive transcription factors through a tight regulation of survival and proliferation pathways.
Is part ofBritish Journal of Nutrition, 2010, vol. 103, p. 168–179
European research projects
Showing items related by title, author, creator and subject.
Activation of Phosphatidylinositol 3-Kinase, but Not Extracellular-Regulated Kinases, Is Necessary to Mediate BrainDerived Neurotrophic Factor-Induced Motoneuron Survival Dolcet Roca, Xavier; Egea Navarro, Joaquim; Soler i Tatché, Rosa Ma.; Martin-Zanca, Dionisio; Comella i Carnicé, Joan Xavier (Wiley, 1999)Chick embryo spinal cord motoneurons develop a trophic response to some neurotrophins when they are maintained in culture in the presence of muscle extract. Thus, after 2 days in culture, brain-derived neurotrophic factor ...
Granado-Serrano, Ana Belén; Martín Garí, Meritxell; Sánchez, V.; Riart Solans, M.; Berdún Hernández, Rebeca; Ludwig, Iziar A.; Rubió Piqué, Laura; Vilaprinyo Terré, Ester; Portero Otín, Manuel; Serrano Casasola, José Carlos Enrique (Nature Research, 2019)Gut microbiota has been suggested to affect lipid metabolism. The objective of this study was to characterize the faecal microbiota signature and both short chain fatty acids (SCFAs) and bile acids (BA) profile of ...
Effect of Pseudomonas graminis strain CPA-7 on the ability of Listeria monocytogenes and Salmonella enterica subsp. enterica to colonize Caco-2 cells after pre-incubation on fresh-cut pear Collazo Cordero, Cyrelys; Abadias i Sero, Mª Isabel; Colás Medà, Pilar; Iglesias Valenzuela, María Belén; Granado-Serrano, Ana Belén; Serrano Casasola, José Carlos Enrique; Viñas Almenar, Inmaculada (Elsevier, 2017)To further gain insight into the mechanism by which the biopreservative bacterium Pseudomonas graminis CPA-7 develops its antimicrobial activity, we have examined the effect that the prior interaction stablished by this ...