| dc.contributor.author | Abecia, Leticia | |
| dc.contributor.author | Balcells Terés, Joaquim | |
| dc.contributor.author | Fondevila, Manuel | |
| dc.contributor.author | Belenguer, Álvaro | |
| dc.contributor.author | Holtrop, G. | |
| dc.contributor.author | Lobley, Gerald E. | |
| dc.date.accessioned | 2018-10-16T08:04:20Z | |
| dc.date.available | 2018-10-16T08:04:20Z | |
| dc.date.issued | 2008 | |
| dc.identifier.issn | 0007-1145 | |
| dc.identifier.uri | http://hdl.handle.net/10459.1/64879 | |
| dc.description.abstract | The contribution of microbial amino acids through caecotrophy to tissue protein metabolism was investigated in lactating does. Attempts were made to
vary microbial supply through a dietary antibiotic, Zn bacitracin, and to vary tissue demand through manipulation of litter size. Three groups of eight
New Zealand does were fed different experimental diets from day 28 of pregnancy to day 26 of lactation. The control group received the basal diet
formulated to meet requirements with grass hay, wheat, soyabean meal and barley grain. The second (no antibiotic) group and the third (bacitracin;
BAC) group ingested the basal diet supplemented with ammonium sulfate (5 g/kg), initially unlabelled (day 1 to day 8) then labelled with 15N
(day 9 to day 30), while the BAC diet was also supplemented throughout with antibiotic (Zn bacitracin; 100 mg/kg). From just after birth each
group of does was subdivided into two groups, each of four females, with the litter size either five (LS5) or nine (LS9) pups. The 15N enrichment in
liver, milk and caecal bacteria amino acids was determined by GC-combustion-isotope ratio MS. All amino acids in bacterial protein were enriched
with the (15NH4)2SO4 treatment, with lysine 15N enrichment significantly greater in caecal bacteria (0·23 (SE 0·0063) atom % excess (ape)) than in
liver (0·04 (SE 0·0004) ape) or milk protein (0·05 (SE 0·0018) ape), confirming the double origin (bacterial and dietary) of tissue lysine. The contribution
of microbes to tissue lysine was 0·23 (SE 0·006) when milk protein was used as reference. | ca_ES |
| dc.description.sponsorship | Work was financed by the Dirección General de Aragón (DGA), through the Research Project reference PM 095/2006. L. A. was funded by a doctoral fellowship (Programa de Formación de Investigadores del Departamento de Educación, Universidades e Investigación del Gobierno Vasco) and by a Marie Curie Training site award (HPMT-CT-2001-00409) during her stage at the Rowett Research Institute. Part of the present study was funded by core grants to the Rowett Research Institute, and Biomathematics and Statistics Scotland (BioSS) by the Scottish Executive. | ca_ES |
| dc.language.iso | eng | ca_ES |
| dc.publisher | Cambridge University Press | ca_ES |
| dc.relation.isformatof | Reproducció del document publicat a https://doi.org/10.1017/S0007114508957986 | ca_ES |
| dc.relation.ispartof | British Journal of Nutrition, 2008, vol. 100, núm. 5, p. 977-983 | ca_ES |
| dc.rights | (c) Cambridge University Press, 2008 | ca_ES |
| dc.subject | Caecotrophy | ca_ES |
| dc.subject | Rabbit does | ca_ES |
| dc.subject | Microbial lysine | ca_ES |
| dc.title | Contribution of gut microbial lysine to liver and milk amino acids in lactating does | ca_ES |
| dc.type | info:eu-repo/semantics/article | ca_ES |
| dc.identifier.idgrec | 014461 | |
| dc.type.version | info:eu-repo/semantics/publishedVersion | ca_ES |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | ca_ES |
| dc.identifier.doi | https://doi.org/10.1017/S0007114508957986 | |
