Contribution of gut microbial lysine to liver and milk amino acids in lactating does
Lobley, Gerald E.
MetadataShow full item record
The contribution of microbial amino acids through caecotrophy to tissue protein metabolism was investigated in lactating does. Attempts were made to vary microbial supply through a dietary antibiotic, Zn bacitracin, and to vary tissue demand through manipulation of litter size. Three groups of eight New Zealand does were fed different experimental diets from day 28 of pregnancy to day 26 of lactation. The control group received the basal diet formulated to meet requirements with grass hay, wheat, soyabean meal and barley grain. The second (no antibiotic) group and the third (bacitracin; BAC) group ingested the basal diet supplemented with ammonium sulfate (5 g/kg), initially unlabelled (day 1 to day 8) then labelled with 15N (day 9 to day 30), while the BAC diet was also supplemented throughout with antibiotic (Zn bacitracin; 100 mg/kg). From just after birth each group of does was subdivided into two groups, each of four females, with the litter size either five (LS5) or nine (LS9) pups. The 15N enrichment in liver, milk and caecal bacteria amino acids was determined by GC-combustion-isotope ratio MS. All amino acids in bacterial protein were enriched with the (15NH4)2SO4 treatment, with lysine 15N enrichment significantly greater in caecal bacteria (0·23 (SE 0·0063) atom % excess (ape)) than in liver (0·04 (SE 0·0004) ape) or milk protein (0·05 (SE 0·0018) ape), confirming the double origin (bacterial and dietary) of tissue lysine. The contribution of microbes to tissue lysine was 0·23 (SE 0·006) when milk protein was used as reference.
Is part ofBritish Journal of Nutrition, 2008, vol. 100, núm. 5, p. 977-983
European research projects
Showing items related by title, author, creator and subject.
Belenguer, Álvaro; Balcells Terés, Joaquim; Guada, J. A.; Decoux, Marc; Milne, Eric (Cambridge University Press, 2005)To study the absorption of microbial lysine in growing rabbits, a labelled diet (supplemented with 15NH4Cl) was administered to six animals (group ISOT); a control group (CTRL, four rabbits) received a similar, but ...
Balcells Terés, Joaquim; Belenguer, Álvaro; Abecia, Leticia; Belanche, Alejandro; Milne, Eric (Elsevier, 2012)In rabbits, caecal fermentation relies to a large extent on the type of substrate available for bacteria. Therefore, in order to study the dietary effect of the source of carbohydrate on microbial nitrogen (N) absorption, ...
Study of the effect of presence or absence of protozoa on rumen fermentation and microbial protein contribution to the chyme Belanche, A.; Abecia, Leticia; Holtrop, G.; Guada, J. A.; Castrillo, C.; de la Fuente Oliver, Gabriel; Balcells Terés, Joaquim (American Society of Animal Science, 2011-06-29)The aim of this study was to investigate the effect of presence or absence of protozoa on rumen fermentation and efficiency of microbial protein synthesis under different diets. Of 20 twin paired lambs, 1 lamb of each pair ...