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dc.contributor.authorCameron Veas, Karla
dc.contributor.authorSolà Ginés, Marc
dc.contributor.authorMoreno, Miguel A.
dc.contributor.authorFraile Sauce, Lorenzo José
dc.contributor.authorMigura Garcia, Lourdes
dc.date.accessioned2018-09-14T10:53:40Z
dc.date.available2018-09-14T10:53:40Z
dc.date.issued2015
dc.identifier.issn0099-2240
dc.identifier.urihttp://hdl.handle.net/10459.1/64716
dc.description.abstractThe aim of this study was to evaluate if the treatments with ceftiofur and amoxicillin are risk factors for the emergence of cephalosporin resistant (CR) E. coli in a pig farm during the rearing period. One hundred 7-day-old piglets were divided into two groups, a control (n = 50) group and a group parenterally treated with ceftiofur (n = 50). During the fattening period, both groups were subdivided in two. A second treatment with amoxicillin was administered in feed to two of the four groups, as follows: group 1 (untreated, n = 20), group 2 (treated with amoxicillin, n = 26), group 3 (treated with ceftiofur, n = 20), and group 4 (treated with ceftiofur and amoxicillin, n = 26). During treatment with ceftiofur, fecal samples were collected before treatment (day 0) and at days 2, 7, 14, 21, and 42 posttreatment, whereas with amoxicillin, the sampling was extended 73 days posttreatment. CR E. coli bacteria were selected on MacConkey agar with ceftriaxone (1 mg/liter). Pulsed-field gel electrophoresis (PFGE), MICs of 14 antimicrobials, the presence of cephalosporin resistance genes, and replicon typing of plasmids were analyzed. Both treatments generated an increase in the prevalence of CR E. coli, which was statistically significant in the treated groups. Resistance diminished after treatment. A total of 47 CR E. coli isolates were recovered during the study period; of these, 15 contained blaCTX-M-1, 10 contained blaCTX-M-14, 4 contained blaCTX-M-9, 2 contained blaCTX-M-15, and 5 contained blaSHV-12. The treatment with ceftiofur and amoxicillin was associated with the emergence of CR E. coli during the course of the treatment. However, by the time of finishing, CR E. coli bacteria were not recovered from the animals.
dc.description.sponsorshipThis work was supported by project AGL2011-28836 from the Ministerio de Economia y Competitividad of Spain. K.C.-V. is a Ph.D. student registered with the Universidad Autonoma de Barcelona.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherAmerican Society for Microbiology
dc.relationMICINN/PN2008-2011/AGL2011-28836
dc.relation.isformatofVersió postprint del document publicat a: https://doi.org/10.1128/AEM.03916-14
dc.relation.ispartofApplied and Environmental Microbiology, 2015, vol. 81, núm. 5, p. 1782-1787
dc.rights(c) American Society for Microbiology, 2015
dc.titleImpact of the use of beta-lactam antimicrobials on the emergence of Escherichia coli resistant to cephalosporins under standard pig rearing conditions
dc.typeinfo:eu-repo/semantics/article
dc.date.updated2018-09-14T10:53:40Z
dc.identifier.idgrec021923
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.identifier.doihttps://doi.org/10.1128/AEM.03916-14


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