Impact of the use of beta-lactam antimicrobials on the emergence of Escherichia coli resistant to cephalosporins under standard pig rearing conditions
Cameron Veas, Karla
Solà Ginés, Marc
Moreno, Miguel A.
Migura Garcia, Lourdes
MetadataShow full item record
The aim of this study was to evaluate if the treatments with ceftiofur and amoxicillin are risk factors for the emergence of cephalosporin resistant (CR) E. coli in a pig farm during the rearing period. One hundred 7-day-old piglets were divided into two groups, a control (n = 50) group and a group parenterally treated with ceftiofur (n = 50). During the fattening period, both groups were subdivided in two. A second treatment with amoxicillin was administered in feed to two of the four groups, as follows: group 1 (untreated, n = 20), group 2 (treated with amoxicillin, n = 26), group 3 (treated with ceftiofur, n = 20), and group 4 (treated with ceftiofur and amoxicillin, n = 26). During treatment with ceftiofur, fecal samples were collected before treatment (day 0) and at days 2, 7, 14, 21, and 42 posttreatment, whereas with amoxicillin, the sampling was extended 73 days posttreatment. CR E. coli bacteria were selected on MacConkey agar with ceftriaxone (1 mg/liter). Pulsed-field gel electrophoresis (PFGE), MICs of 14 antimicrobials, the presence of cephalosporin resistance genes, and replicon typing of plasmids were analyzed. Both treatments generated an increase in the prevalence of CR E. coli, which was statistically significant in the treated groups. Resistance diminished after treatment. A total of 47 CR E. coli isolates were recovered during the study period; of these, 15 contained blaCTX-M-1, 10 contained blaCTX-M-14, 4 contained blaCTX-M-9, 2 contained blaCTX-M-15, and 5 contained blaSHV-12. The treatment with ceftiofur and amoxicillin was associated with the emergence of CR E. coli during the course of the treatment. However, by the time of finishing, CR E. coli bacteria were not recovered from the animals.
Is part ofApplied and Environmental Microbiology, 2015, vol. 81, núm. 5, p. 1782-1787
European research projects
Showing items related by title, author, creator and subject.
Shedding of cephalosporin resistant Escherichia coli in pigs from conventional farms after early treatment with antimicrobials Cameron Veas, Karla; Moreno, Miguel A.; Fraile Sauce, Lorenzo José; Migura Garcia, Lourdes (Elsevier, 2016)This study assessed the dynamics of cephalosporin resistant (CR) E. coli populations during the life cycle of pigs treated early in life with ceftiofur or tulathromycin. The study was conducted at eight conventional pig ...
Antimicrobial resistance of zoonotic and commensal bacteria in Europe: the missing link between consumption and resistance in veterinary medicine Garcia Migura, Lourdes; Hendriksen, Rene S.; Fraile Sauce, Lorenzo José; Aarestrup, Frank M. (Elsevier, 2014)The emergence of resistance in food animals has been associated to the consumption of antimicrobials in veterinary medicine. Consequently, monitoring programs have been designed to monitor the occurrence of antimicrobial ...
Multidrug resistant Salmonella enterica isolated from conventional pig farms using antimicrobial agents in preventative medicine programmes Cameron Veas, Karla; Fraile Sauce, Lorenzo José; Napp Avelli, Sebastián; Garrido, Victoria; Grilló, María Jesús; Migura Garcia, Lourdes (Elsevier, 2018)A longitudinal study was conducted to investigate the presence of multidrug antimicrobial resistance (multi-AR) in Salmonella enterica in pigs reared under conventional preventative medicine programmes in Spain and the ...