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dc.contributor.authorBohórquez, José Alejandro
dc.contributor.authorDefaus, Sira
dc.contributor.authorMuñoz González, Sara
dc.contributor.authorPerez-Simó, Marta
dc.contributor.authorRosell, Rosa
dc.contributor.authorFraile Sauce, Lorenzo José
dc.contributor.authorSobrino, Francisco
dc.contributor.authorAndreu, David
dc.contributor.authorGanges, Llilianne
dc.date.accessioned2018-09-05T11:43:15Z
dc.date.available2018-09-05T11:43:15Z
dc.date.issued2017
dc.identifier.issn0168-1702
dc.identifier.urihttp://hdl.handle.net/10459.1/64676
dc.description.abstractThree dendrimeric peptides were synthesized in order to evaluate their immunogenicity and their potential protection against classical swine fever virus (CSFV) in domestic pigs. Construct 1, an optimized version of a previously used dendrimer, had four copies of a B-cell epitope derived from CSFV E2 glycoprotein connected to an also CSFV-derived T-cell epitope through maleimide instead of thioether linkages. Construct 2 was similarly built but included only two copies of the B-cell epitope, and in also bivalent construct 3 the CSFV T-cell epitope was replaced by a previously described one from the 3A protein of foot-and-mouth disease virus (FMDV). Animals were inoculated twice with a 21-day interval and challenged 15days after the second immunization. Clinical signs were recorded daily and ELISA tests were performed to detect antibodies against specific peptide and E2. The neutralising antibody response was assessed 13days after challenge. Despite the change to maleimide connectivity, only partial protection against CSFV was again observed. The best clinical protection was observed in group 3. Animals inoculated with constructs 2 and 3 showed higher anti-peptide humoral response, suggesting that two copies of the B-cell epitope are sufficient or even better than four copies for swine immune recognition. In addition, for construct 3 higher neutralizing antibody titres against CSFV were detected. Our results support the immunogenicity of the CSFV B-cell epitope and the cooperative role of the FMDV 3A T-cell epitope in inducing a neutralising response against CSFV in domestic pigs. This is also the first time that the FMDV T-cell epitope shows effectivity in improving swine immune response against a different virus. Our findings highlight the relevance of dendrimeric peptides as a powerful tool for epitope characterization and antiviral strategies development.
dc.description.sponsorshipThe research in CReSA was supported by grant AGL2015-66907 from the Spanish government. J.A. B. had a pre-doctoralfellowship FPI-MINECO 2016 from Spanish government. S. M.had a pre-doctoral fellowship FI-DGR 2014 from AGAUR, Gen-eralitat de Catalunya. Work at CBMSO was supported by grantsAGL2014-52395-C2-01 (MINECO, Spain) and S2013/ABI-2906-PLATESA (Comunidad Autónoma de Madrid). Work at UPF wasfunded by AGL2014-52395-C2-02 (MINECO, Spain).
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherElsevier
dc.relationMINECO/PN2013-2016/AGL2015-66907
dc.relationMINECO/PN2013-2016/AGL2014-52395-C2-01
dc.relationMINECO/PN2013-2016/AGL2014-52395-C2-02
dc.relation.isformatofVersió postprint del document publicat a: https://doi.org/10.1016/j.virusres.2017.05.020
dc.relation.ispartofVirus Research, 2017, vol. 238, p. 8-12
dc.rightscc-by-nc-nd, (c) Elsevier, 2017
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectB cell epitope
dc.subjectCSFV
dc.subjectDendrimeric peptide
dc.subjectFMDV
dc.titleA bivalent dendrimeric peptide bearing a T-cell epitope from foot-and-mouth disease virus protein 3A improves humoral response against classical swine fever virus
dc.typeinfo:eu-repo/semantics/article
dc.date.updated2018-09-05T11:43:15Z
dc.identifier.idgrec025658
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.identifier.doihttps://doi.org/10.1016/j.virusres.2017.05.020


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cc-by-nc-nd, (c) Elsevier, 2017
Except where otherwise noted, this item's license is described as cc-by-nc-nd, (c) Elsevier, 2017