Towards the engineering of the monoterpene secoiridoid pathway in transgenic tobacco plants
Miralpeix i Anglada, Bruna
Universitat de Lleida. Departament de Producció Vegetal i Ciència Forestal
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Monoterpene secoiridoid biosynthesis contributes the terpene component of terpenoid indole alkaloids produced in the medicinal plant Catharanthus roseus. The thesis focuses on the development of fundamental knowledge and methodology to reconstruct the monoterpene secoiridoid pathway in tobacco plants using metabolic engineering. The thrust of the thesis concentrated on remaining challenges to engineer complex secondary metabolic pathways in plants. We focused on the early part of the pathway and we recovered a population of transgenic tobacco plants expressing different combinations of transgenes. Analysis at metabolomic and transcriptomic levels provided novel insights into remaining bottlenecks for the effective engineering of secondary metabolic pathways in plants. The experimental part of the thesis is complemented by the development of a systematic patent search to create an IP database with all relevant patents related with the engineering of terpenoid indole alkaloid pathway in plants.
European research projects
- Tesis Doctorals 
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Miralpeix i Anglada, Bruna; Rischer, Heiko; Häkkinen, Suvi T.; Ritala, Anneli; Seppänen-Laakso, Tuulikki; Oksman-Caldentey, Kirsi-Marja; Capell Capell, Teresa; Christou, Paul (Bentham Science Publishers, 2013)Secondary products are small molecular weight compounds produced by secondary metabolic pathways in plants. They are re- garded as non-essential for normal growth and development but often confer benefits such as defense ...
Strategic patent analysis in plant biotechnology: terpenoid indole alkaloid metabolic engineering as a case study Miralpeix i Anglada, Bruna; Sabalza Gallués, Maite; Twyman, Richard M.; Capell Capell, Teresa; Christou, Paul (John Wiley & Sons Ltd, 2014)The do-it-yourself patent search is a useful alternative to professional patent analysis particularly in the context of publicly funded projects where funds for IP activities may be limited. As a case study, we analysed ...
Zorrilla López, Uxue; Masip Vilà, Gemma; Arjó Pont, Gemma; Bai, Chao; Banakar, Raviraj; Bassie Rene, Ludovic; Berman Quintana, Judit; Farré Martinez, Gemma; Miralpeix i Anglada, Bruna; Pérez Massot, Eduard; Sabalza Gallués, Maite; Sanahuja Solsona, Georgina; Vamvaka, Evangelia; Twyman, Richard M.; Christou, Paul; Zhu, Changfu; Capell Capell, Teresa (University of the Basque Country Press, 2013)Metabolic engineering in plants can be used to increase the abundance of specific valuable metabolites, but single-point interventions generally do not improve the yields of target metabolites unless that product is ...