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dc.contributor.authorGotor Vila, Amparo María
dc.contributor.authorUsall i Rodié, Josep
dc.contributor.authorTorres Sanchis, Rosario
dc.contributor.authorAbadias i Sero, Mª Isabel
dc.contributor.authorTeixidó i Espasa, Neus
dc.date.accessioned2018-04-18T07:19:00Z
dc.date.available2018-04-18T07:19:00Z
dc.date.issued2017
dc.identifier.issn1386-6141
dc.identifier.urihttp://hdl.handle.net/10459.1/63111
dc.description.abstractThe present work focuses on the assessment and comparison of three different formulation technologies and the effect of protectants on cell viability, storage stability and antagonistic activity of the biocontrol agent Bacillus amyloliquefaciens CPA-8. Cultures were concentrated with different protective substances such as MgSO4, sucrose and skimmed milk (SM) and subjected to liquid formulation, freeze-drying and fluid-bed spray-drying. Results showed that CPA-8 freeze-dried cells without protectants or amended with SM suffered the highest losses in cell viability (0.41−0.48 log). Moreover, the cell viability of the tested freeze-dried products decreased after four months of storage at both tested temperatures (4 and 22 °C). Otherwise, liquid and fluid-bed spray-dried products were stable for four months at 4 °C and for 12 months at 22, 4 and −20 °C, respectively, and no effect of the protectants was observed. The most suitable CPA-8 products were then tested against Monilinia laxa and M. fructicola in artificially wounded nectarines and in all cases the antagonistic activity was maintained similar to fresh cells. The efficacy results revealed that the formulation process did not affect the biocontrol potential of CPA-8. This work led us to conclude that effective formulations with final concentrations ranging from 1.93 × 109–2.98 × 109 CFU ml−1 and from 4.76 × 109–1.03 × 1010 CFU g−1 were obtained for liquid and dried products, respectively. Additionally, the suitability of the fluid-bed spray drying technology should be taken into account to develop a stable and effective CPA-8 product for practical applications to control brown rot in stone fruit.ca_ES
dc.description.sponsorshipThis research was supported by the European project BIOCOMES FP7-612713 and by the Secretaria d’Universitats i Recerca del Departament d’Economia i Coneixement de la Generalitat de Catalunya for the PhD Grant 2014-FI-B00367 (Amparo M. Gotor Vila). The authors also thank CERCA Program (Generalitat de Catalunya).ca_ES
dc.language.isoengca_ES
dc.publisherSpringer Verlagca_ES
dc.publisherInternational Organization for Biological Controlca_ES
dc.relation.isformatofVersió postprint del document publicat a https://doi.org/10.1007/s10526-017-9802-3ca_ES
dc.relation.ispartofBioControl, 2017, vol. 62, núm. 4, p. 545–555ca_ES
dc.rights(c) International Organization for Biological Control (IOBC), 2017ca_ES
dc.subjectBacillus spp.ca_ES
dc.subjectProtectantsca_ES
dc.subjectShelf-lifeca_ES
dc.subjectMonilinia spp.ca_ES
dc.subjectBiocontrol efficacyca_ES
dc.titleFormulation of the biocontrol agent Bacillus amyloliquefaciens CPA-8 using different approaches: liquid, freeze-drying and fluid-bed spray-dryingca_ES
dc.typearticleca_ES
dc.identifier.idgrec030136
dc.type.versionacceptedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.identifier.doihttps://doi.org/10.1007/s10526-017-9802-3
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/612713ca_ES


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