Formulation of the biocontrol agent Bacillus amyloliquefaciens CPA-8 using different approaches: liquid, freeze-drying and fluid-bed spray-drying
Gotor Vila, Amparo María
Torres Sanchis, Rosario
MetadataShow full item record
The present work focuses on the assessment and comparison of three different formulation technologies and the effect of protectants on cell viability, storage stability and antagonistic activity of the biocontrol agent Bacillus amyloliquefaciens CPA-8. Cultures were concentrated with different protective substances such as MgSO4, sucrose and skimmed milk (SM) and subjected to liquid formulation, freeze-drying and fluid-bed spray-drying. Results showed that CPA-8 freeze-dried cells without protectants or amended with SM suffered the highest losses in cell viability (0.41−0.48 log). Moreover, the cell viability of the tested freeze-dried products decreased after four months of storage at both tested temperatures (4 and 22 °C). Otherwise, liquid and fluid-bed spray-dried products were stable for four months at 4 °C and for 12 months at 22, 4 and −20 °C, respectively, and no effect of the protectants was observed. The most suitable CPA-8 products were then tested against Monilinia laxa and M. fructicola in artificially wounded nectarines and in all cases the antagonistic activity was maintained similar to fresh cells. The efficacy results revealed that the formulation process did not affect the biocontrol potential of CPA-8. This work led us to conclude that effective formulations with final concentrations ranging from 1.93 × 109–2.98 × 109 CFU ml−1 and from 4.76 × 109–1.03 × 1010 CFU g−1 were obtained for liquid and dried products, respectively. Additionally, the suitability of the fluid-bed spray drying technology should be taken into account to develop a stable and effective CPA-8 product for practical applications to control brown rot in stone fruit.
Is part ofBioControl, 2017, vol. 62, núm. 4, p. 545–555
European research projects
Showing items related by title, author, creator and subject.
Enhanced shelf-life of the formulated biocontrol agent Bacillus amyloliquefaciens CPA-8 combining diverse packaging strategies and storage conditions Gotor Vila, Amparo María; Usall i Rodié, Josep; Torres Sanchis, Rosario; Solsona Aixalà, Cristina; Teixidó i Espasa, Neus (Elsevier, 2019-02-02)Two effective biocontrol products (named as BA3 and BA4) based on Bacillus amyloliquefaciens CPA-8 have been reported as a potential alternative to chemical applications against brown rot caused by Monilinia spp. on stone ...
Biocontrol products based on Bacillus amyloliquefaciens CPA-8 using fluid-bed spray-drying process to control postharvest brown rot in stone fruit Gotor Vila, Amparo María; Usall i Rodié, Josep; Torres Sanchis, Rosario; Solsona Aixalà, Cristina; Teixidó i Espasa, Neus (Elsevier, 2017)Two products based on the biocontrol agent Bacillus amyloliquefaciens CPA-8 have been developed as an effective alternative to chemical applications to control postharvest brown rot in stone fruit. As part of the production ...
Biological characterization of the biocontrol agent Bacillus amyloliquefaciens CPA-8: the effect of temperature, pH and water activity on growth, susceptibility to antibiotics and detection of enterotoxic genes Gotor Vila, Amparo María; Teixidó i Espasa, Neus; Sisquella Sanagustín, María; Torres Sanchis, Rosario; Usall i Rodié, Josep (Springer Verlag, 2017)This work focuses on the biological understanding of the biocontrol agent Bacillus amyloliquefaciens CPA-8 in order to accomplish the characterization required in the registration process for the development of a ...