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dc.contributor.authorGotor Vila, Amparo María
dc.contributor.authorTeixidó i Espasa, Neus
dc.contributor.authorDi Francesco, A.
dc.contributor.authorUsall i Rodié, Josep
dc.contributor.authorUgolini, L.
dc.contributor.authorTorres Sanchis, Rosario
dc.contributor.authorMari, M.
dc.date.accessioned2018-04-17T12:12:31Z
dc.date.available2018-04-17T12:12:31Z
dc.date.issued2017
dc.identifier.issn0740-0020
dc.identifier.urihttp://hdl.handle.net/10459.1/63110
dc.description.abstractThe present work focuses on the antifungal effect of volatile organic compounds (VOCs) produced by Bacillus amyloliquefaciens CPA-8 against Monilinia laxa, M. fructicola and Botrytis cinera, three postharvest fruit pathogens of sweet cherry fruit. VOCs were evaluated with a double petri dish assay against mycelial and colony growth of target pathogens. For this purpose, CPA-8 was grown on different media and cultured for 24 and 48 h at 30 °C before assays. Data showed that mycelial growth inhibition was higher when CPA-8 was grown on Tryptone Soya Agar (TSA) while no differences were generally observed when CPA-8 was cultured for either, 24 and 48 h. Moreover, no effects were observed on colony growth. The main volatile compounds emitted by CPA-8 were identified by solid-phase microextraction (SPME)-gas chromatography as 1,3 pentadiene, acetoin (3-hydroxy-2-butanone) and thiophene. Pure compounds were also tested in vitro on mycelial growth inhibition and their EC50 values against the three pathogens were estimated. Thiophene was the most effective VOC, showing more than 82% suppression of mycelial growth at the highest concentration (1.35 μL/mL headspace) and EC50 values ranging from 0.06 to 6.67 μL/mL headspace. Finally, the effectiveness of thiophene and CPA-8 VOCs was evaluated against artificially inoculated cherry fruits. Among the target pathogens, M. fructicola was clearly controlled by CPA-8 with less than 25% of rotten fruits compared to the control (65% disease incidence) and for all pathogens, less than 37.5% of CPA-8 treated decayed fruits produced spores (disease sporulation). Otherwise, pure thiophene showed no effect against any pathogen on disease incidence and disease sporulation. The results indicated that VOCs produced by B. amyloliquefaciens CPA-8 could develop an additive antifungal effect against postharvest fruit pathogens on stone fruit.ca_ES
dc.description.sponsorshipThis research was supported by the European project BIOCOMES FP7-612713 and by the Catalan government (Generalitat de Catalunya) for the PhD grant 2016-FI-B2 00143 (Amparo M. Gotor).ca_ES
dc.language.isoengca_ES
dc.publisherElsevierca_ES
dc.relation.isformatofVersió postprint del document publicat a https://doi.org/10.1016/j.fm.2017.01.006ca_ES
dc.relation.ispartofFood Microbiology, 2017, vol. 64, p. 219-225ca_ES
dc.rightscc-by-nc-nd (c) Elsevier, 2017ca_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectBiocontrolca_ES
dc.subjectBacillus spp.ca_ES
dc.subjectMonilinia spp.ca_ES
dc.subjectBotrytis spp.ca_ES
dc.subjectVOCsca_ES
dc.subjectCherryca_ES
dc.titleAntifungal effect of volatile organic compounds produced by Bacillus amyloliquefaciens CPA-8 against fruit pathogen decays of cherryca_ES
dc.typearticleca_ES
dc.type.versionacceptedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.identifier.doihttps://doi.org/10.1016/j.fm.2017.01.006
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/612713ca_ES


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cc-by-nc-nd (c) Elsevier, 2017
Except where otherwise noted, this item's license is described as cc-by-nc-nd (c) Elsevier, 2017