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dc.contributor.authorGotor Vila, Amparo María
dc.contributor.authorTeixidó i Espasa, Neus
dc.contributor.authorUsall i Rodié, Josep
dc.contributor.authorDashevskaya, Svetlana
dc.contributor.authorTorres Sanchis, Rosario
dc.date.accessioned2018-04-17T10:33:19Z
dc.date.available2018-04-17T10:33:19Z
dc.date.issued2016
dc.identifier.issn0003-4746
dc.identifier.urihttp://hdl.handle.net/10459.1/63108
dc.description.abstractIn this work, reliable tools were developed to detect and identify the biocontrol strain CPA-8 using DNA amplification techniques. As a first approach, the RAPD (random amplified polymorphic DNA) technique was applied to a collection of 77 related Bacillus species. Among the primers tested, the primer pair OPG1/OPG6 amplified a 668 bp specific product to the strain CPA-8 that was sequenced and used to design SCAR (sequence-characterised amplified regions) primer pairs. The SCAR-4 marker amplified a semi-specific fragment of 665 bp not only for the strain CPA-8 but also for other 12 strains whose morphology was completely different from CPA-8. Another approach was developed to obtain a strain-specific genomic marker related to ecological adaptations of Bacillus amyloliquefaciens species. The primer pair F2/R2 obtained from RBAM 007760, a gene involved in surface adhesion, amplified a 265 bp fragment unique for strain CPA-8. Our results revealed that these two molecular markers, SCAR-4 and RBAM 007760 F2/R2 provide suitable monitoring tools to specifically identify the biocontrol CPA-8 when applied against brown rot caused by Monilinia spp. in stone fruit. Moreover, our findings demonstrate that the strain CPA-8 is affiliated with B. amyloliquefaciens species that was formerly designated as Bacillus subtilis.ca_ES
dc.description.sponsorshipThis research was supported by the European project BIOCOMES FP7-612713 and by the Catalan government (Generalitat de Catalunya) for the PhD grant 2015-FI-B100166 (Amparo M. Gotor). No conflict of interest declared.ca_ES
dc.language.isoengca_ES
dc.publisherWileyca_ES
dc.publisherAssociation of Applied Biologistsca_ES
dc.relation.isformatofVersió postprint del document publicat a https://doi.org/10.1111/aab.12298ca_ES
dc.relation.ispartofAnnals of Applied Biology, 2016, vol. 169, núm. 2, p. 248–256ca_ES
dc.rights© Association of Applied Biologists, 2016ca_ES
dc.subjectBacillus sppca_ES
dc.subjectBiological controlca_ES
dc.subjectMolecular markerca_ES
dc.subjectMonitoringca_ES
dc.subjectSCARca_ES
dc.titleDevelopment of a SCAR marker and a strain-specific genomic marker for the detection of the biocontrol agent strain CPA-8 Bacillus amyloliquefaciens (formerly B. subtilis)ca_ES
dc.typearticleca_ES
dc.type.versionacceptedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.identifier.doihttps://doi.org/10.1111/aab.12298
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/612713ca_ES


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