Development of a SCAR marker and a strain-specific genomic marker for the detection of the biocontrol agent strain CPA-8 Bacillus amyloliquefaciens (formerly B. subtilis)
Gotor Vila, Amparo María
Torres Sanchis, Rosario
MetadataShow full item record
In this work, reliable tools were developed to detect and identify the biocontrol strain CPA-8 using DNA amplification techniques. As a first approach, the RAPD (random amplified polymorphic DNA) technique was applied to a collection of 77 related Bacillus species. Among the primers tested, the primer pair OPG1/OPG6 amplified a 668 bp specific product to the strain CPA-8 that was sequenced and used to design SCAR (sequence-characterised amplified regions) primer pairs. The SCAR-4 marker amplified a semi-specific fragment of 665 bp not only for the strain CPA-8 but also for other 12 strains whose morphology was completely different from CPA-8. Another approach was developed to obtain a strain-specific genomic marker related to ecological adaptations of Bacillus amyloliquefaciens species. The primer pair F2/R2 obtained from RBAM 007760, a gene involved in surface adhesion, amplified a 265 bp fragment unique for strain CPA-8. Our results revealed that these two molecular markers, SCAR-4 and RBAM 007760 F2/R2 provide suitable monitoring tools to specifically identify the biocontrol CPA-8 when applied against brown rot caused by Monilinia spp. in stone fruit. Moreover, our findings demonstrate that the strain CPA-8 is affiliated with B. amyloliquefaciens species that was formerly designated as Bacillus subtilis.
Is part ofAnnals of Applied Biology, 2016, vol. 169, núm. 2, p. 248–256
European research projects
Showing items related by title, author, creator and subject.
Biological characterization of the biocontrol agent Bacillus amyloliquefaciens CPA-8: the effect of temperature, pH and water activity on growth, susceptibility to antibiotics and detection of enterotoxic genes Gotor Vila, Amparo María; Teixidó i Espasa, Neus; Sisquella Sanagustín, María; Torres Sanchis, Rosario; Usall i Rodié, Josep (Springer Verlag, 2017)This work focuses on the biological understanding of the biocontrol agent Bacillus amyloliquefaciens CPA-8 in order to accomplish the characterization required in the registration process for the development of a ...
Environmental fate and behaviour of the biocontrol agent Bacillus amyloliquefaciens CPA‐8 after preharvest application to stone fruit Vilanova Torren, Laura; Teixidó i Espasa, Neus; Usall i Rodié, Josep; Balsells Llauradó, Marta; Gotor Vila, Amparo María; Torres Sanchis, Rosario (WileySociety of Chemical Industry, 2018)BACKGROUND: Bacillus amyloliquefaciens strain CPA‐8 has been described as an effective biocontrol agent to control brown rot in stone fruit for both preharvest and postharvest applications. However, no information about ...
Formulation of the biocontrol agent Bacillus amyloliquefaciens CPA-8 using different approaches: liquid, freeze-drying and fluid-bed spray-drying Gotor Vila, Amparo María; Usall i Rodié, Josep; Torres Sanchis, Rosario; Abadias i Sero, Mª Isabel; Teixidó i Espasa, Neus (Springer VerlagInternational Organization for Biological Control, 2017)The present work focuses on the assessment and comparison of three different formulation technologies and the effect of protectants on cell viability, storage stability and antagonistic activity of the biocontrol agent ...