Universitat de Lleida
    • English
    • català
    • español
  • català 
    • English
    • català
    • español
  • Inicia la sessió
Repositori Obert UdL
Visualitza l'element 
  •   Inici
  • Recerca
  • Producció Vegetal i Ciència Forestal
  • Articles publicats (Producció Vegetal i Ciència Forestal)
  • Visualitza l'element
  •   Inici
  • Recerca
  • Producció Vegetal i Ciència Forestal
  • Articles publicats (Producció Vegetal i Ciència Forestal)
  • Visualitza l'element
JavaScript is disabled for your browser. Some features of this site may not work without it.

Mushroom emergence detected by combining spore trapping with molecular techniques

Thumbnail
Visualitza/Obre
Postprint (4.625Mb)
Data de publicació
2017
Autor/a
Castaño Soler, Carles
Oliva Palau, Jonàs
Martínez de Aragón, Juan
Alday, Josu G.
Parladé Izquierdo, Xavier
Pera i Álvarez, Joan
Bonet Lledos, José Antonio
Citació recomanada
Castaño Soler, Carles; Oliva Palau, Jonàs; Martínez de Aragón, Juan; Alday, Josu G.; Parladé Izquierdo, Xavier; Pera i Álvarez, Joan; Bonet Lledos, José Antonio; . (2017) . Mushroom emergence detected by combining spore trapping with molecular techniques. Applied and Environmental Microbiology, 2017, vol. 83, núm. 13, p. e00600-17. https://doi.org/10.1128/AEM.00600-17.
Impacte


Logo de Web of Science    citacions a Web of Science

Logo d'Scopus    citacions a Scopus

Logo de Google Acadèmic  Google Acadèmic
Compartir
Exportar a Mendeley
Metadades
Mostra el registre d'unitat complet
Resum
ABSTRACT Obtaining reliable and representative mushroom production data requires time-consuming sampling schemes. In this paper, we assessed a simple methodology to detect mushroom emergence by trapping the fungal spores of the fruiting body community in plots where mushroom production was determined weekly. We compared the performance of filter paper traps with that of funnel traps and combined these spore trapping methods with species-specific quantitative real-time PCR and Illumina MiSeq to determine the spore abundance. Significantly more MiSeq proportional reads were generated for both ectomycorrhizal and saprotrophic fungal species using filter traps than were obtained using funnel traps. The spores of 37 fungal species that produced fruiting bodies in the study plots were identified. Spore community composition changed considerably over time due to the emergence of ephemeral fruiting bodies and rapid spore deposition (lasting from 1 to 2 weeks), which occurred in the absence of rainfall events. For many species, the emergence of epigeous fruiting bodies was followed by a peak in the relative abundance of their airborne spores. There were significant positive relationships between fruiting body yields and spore abundance in time for five of seven fungal species. There was no relationship between fruiting body yields and their spore abundance at plot level, indicating that some of the spores captured in each plot were arriving from the surrounding areas. Differences in fungal detection capacity by spore trapping may indicate different dispersal ability between fungal species. Further research can help to identify the spore rain patterns for most common fungal species. IMPORTANCE Mushroom monitoring represents a serious challenge in economic and logistical terms because sampling approaches demand extensive field work at both the spatial and temporal scales. In addition, the identification of fungal taxa depends on the expertise of experienced fungal taxonomists. Similarly, the study of fungal dispersal has been constrained by technological limitations, especially because the morphological identification of spores is a challenging and time-consuming task. Here, we demonstrate that spores from ectomycorrhizal and saprotrophic fungal species can be identified using simple spore traps together with either MiSeq fungus-specific amplicon sequencing or species-specific quantitative real-time PCR. In addition, the proposed methodology can be used to characterize the airborne fungal community and to detect mushroom emergence in forest ecosystems.
URI
http://hdl.handle.net/10459.1/63106
DOI
https://doi.org/10.1128/AEM.00600-17
És part de
Applied and Environmental Microbiology, 2017, vol. 83, núm. 13, p. e00600-17
Projectes de recerca europeus
Col·leccions
  • Publicacions de projectes de recerca del Plan Nacional [2223]
  • Articles publicats (Agrotecnio Center) [954]
  • Articles publicats (Producció Vegetal i Ciència Forestal) [654]

Publicacions relacionades

Mostrant elements relacionats per títol, autor i matèria.

  • Rainfall homogenizes while fruiting increases diversity of spore deposition in Mediterranean conditions 

    Castaño Soler, Carles; Bonet Lledos, José Antonio; Oliva Palau, Jonàs; Farré Martinez, Gemma; Martínez de Aragón, Juan; Parladé, Javier; Pera i Álvarez, Joan; Alday, Josu G. (ElsevierBritish Mycological Society, 2019-09-01)
    There is a lack of knowledge regarding the main factors modulating fungal spore deposition in forest ecosystems. We have described the local spatio-temporal dynamics of fungal spore deposition along a single fruiting season ...
  • Insect defoliation is linked to a decrease in soil ectomycorrhizal biomass and shifts in needle endophytic communities 

    Castaño Soler, Carles; Camarero Martínez, Jesús Julio; Zas, Rafael; Sampedro, Luis; Bonet Lledos, José Antonio; Alday, Josu G.; Oliva Palau, Jonàs (Oxford University Press, 2020-08-12)
    Insect outbreaks of increasing frequency and severity in forests are predicted due to climate change. Insect herbivory is known to promote physiological changes in forest trees. However, little is known about whether these ...
  • Soil drying procedure affects the DNA quantification of Lactarius vinosus but does not change the fungal community composition 

    Castaño Soler, Carles; Parladé Izquierdo, Xavier; Pera i Álvarez, Joan; Martínez de Aragón, Juan; Alday, Josu G.; Bonet Lledos, José Antonio (Springer-Verlag, 2016)
    Drying soil samples before DNA extraction is commonly used for specific fungal DNA quantification and metabarcoding studies, but the impact of different drying procedures on both the specific fungal DNA quantity and the ...

Contacteu amb nosaltres | Envia comentaris | Avís legal
© 2021 BiD. Universitat de Lleida
Metadades subjectes a 
 

 

Explora

Tot el repositoriComunitats i col·leccionsPer data d'edicióAutorsTítolsMatèriesAquesta col·leccióPer data d'edicióAutorsTítolsMatèries

Estadístiques

Veure estadístiques d'ús

D'interès

Política institucional d'accés obertDiposita les teves publicacionsDiposita dades de recercaSuport a la recerca

Contacteu amb nosaltres | Envia comentaris | Avís legal
© 2021 BiD. Universitat de Lleida
Metadades subjectes a