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dc.contributor.authorGonzález Arias, Cyndia A.
dc.contributor.authorMarín Sillué, Sònia
dc.contributor.authorRojas-García, A. E.
dc.contributor.authorSanchís Almenar, Vicente
dc.contributor.authorRamos Girona, Antonio J.
dc.date.accessioned2018-04-04T11:00:51Z
dc.date.available2018-09-06T22:18:46Z
dc.date.issued2017-09-19
dc.identifier.issn0278-6915
dc.identifier.urihttp://hdl.handle.net/10459.1/62976
dc.description.abstractOchatoxin A (OTA) is one of the most important mycotoxins based on its toxicity. The oral route is the main gateway of entry of OTA into the human body, and specialized epithelial cells constitute the first barrier. The present study investigated the in vitro cytotoxic effect of OTA (5, 15 and 45 μM) and production of OTA metabolities in Caco-2 and HepG2 cells using a co-culture Transwell System to mimic the passage through the intestinal epithelium and hepatic metabolism. The results derived from MTS cell viability assays and transepithelial electrical resistance measurements showed that OTA was slightly cytotoxic at the lowest concentration at 3 h, but significant toxicity was observed at all concentrations at 24 h. OTA metabolites generated in this co-culture were ochratoxin B (OTB), OTA methyl ester, OTA ethyl ester and the OTA glutathione conjugate (OTA-GSH). OTA methyl ester was the major metabolite found in both Caco-2 and HepG2 cells after all treatments. Our results showed that OTA can cause cell damage through several mechanisms and that the OTA exposure time is more important that the dosage in in vitro studies. OTA methyl ester is proposed as an OTA exposure biomarker, although future studies should be conducted.
dc.description.sponsorshipThe authors are grateful to the Spanish (Project AGL2011-24862) and Catalonian (XaRTA-Reference Network on Food Technology) Governments for their financial support. C.A. González-Arias thanks the Secretaria de Universitats i Recerca del Departament de Economia i Coneixement of the Generalitat de Catalunya for the pre-doctoral grant.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherElsevier
dc.relationMICINN/PN2008-2011/AGL2011-24862
dc.relation.isformatofVersió postprint del document publicat a: https://doi.org/10.1016/j.fct.2017.09.011
dc.relation.ispartofFood and Chemical Toxicology, 2017, vol. 109, p. 333-340
dc.rightscc-by-nc-nd, (c) Elsevier, 2017
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectOchratoxin A
dc.subjectCytotoxicity
dc.subjectOTA
dc.subjectBiotransformation
dc.titleUPLC-MS/MS analysis of ochratoxin A metabolites produced by Caco-2 and HepG2 cells in a co-culture system
dc.typeinfo:eu-repo/semantics/article
dc.date.updated2018-04-04T11:00:51Z
dc.identifier.idgrec025966
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.identifier.doihttps://doi.org/10.1016/j.fct.2017.09.011


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cc-by-nc-nd, (c) Elsevier, 2017
Except where otherwise noted, this item's license is described as cc-by-nc-nd, (c) Elsevier, 2017