Quantitative operating principles of yeast metabolism during adaptation to heat stress

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2018Author
Pereira, Tania
Salvadó López, Baldiri
Altés, Gisela
Suggested citation
Pereira, Tania;
Vilaprinyo Terré, Ester;
Bellí i Martínez, Gemma;
Herrero Perpiñán, Enrique;
Salvadó López, Baldiri;
Sorribas Tello, Albert;
...
Alves, Rui.
(2018)
.
Quantitative operating principles of yeast metabolism during adaptation to heat stress.
Cell Reports, 2018, vol. 22, núm. 9, p. 2421-2430.
https://doi.org/10.1016/j.celrep.2018.02.020.
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Microorganisms evolved adaptive responses to survive stressful challenges in ever-changing environments. Understanding the relationships between the physiological/metabolic adjustments allowing cellular stress adaptation and gene expression changes being used by organisms to achieve such adjustments may significantly impact our ability to understand and/or guide evolution. Here, we studied those relationships during adaptation to various stress challenges in Saccharomyces cerevisiae, focusing on heat stress responses. We combined dozens of independent experiments measuring whole-genome gene expression changes during stress responses with a simplified kinetic model of central metabolism. We identified alternative quantitative ranges for a set of physiological variables in the model (production of ATP, trehalose, NADH, etc.) that are specific for adaptation to either heat stress or desiccation/rehydration. Our approach is scalable to other adaptive responses and could assist in developing biotechnological applications to manipulate cells for medical, biotechnological, or synthetic biology purposes.
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Cell Reports, 2018, vol. 22, núm. 9, p. 2421-2430European research projects
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