Quantitative operating principles of yeast metabolism during adaptation to heat stress

Pereira, Tania; Vilaprinyo Terré, Ester; Bellí i Martínez, Gemma; Herrero Perpiñán, Enrique; Salvadó López, Baldiri; Sorribas Tello, Albert; Altés, Gisela; Alves, Rui

doi:https://doi.org/10.1016/j.celrep.2018.02.020

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2018

Author

Pereira, Tania

Vilaprinyo Terré, Ester

Bellí i Martínez, Gemma

Herrero Perpiñán, Enrique

Salvadó López, Baldiri

Sorribas Tello, Albert

Altés, Gisela

Alves, Rui

Suggested citation

Pereira, Tania; Vilaprinyo Terré, Ester; Bellí i Martínez, Gemma; Herrero Perpiñán, Enrique; Salvadó López, Baldiri; Sorribas Tello, Albert; ... Alves, Rui. (2018) . Quantitative operating principles of yeast metabolism during adaptation to heat stress. Cell Reports, 2018, vol. 22, núm. 9, p. 2421-2430. https://doi.org/10.1016/j.celrep.2018.02.020.

Abstract

Microorganisms evolved adaptive responses to survive stressful challenges in ever-changing environments. Understanding the relationships between the physiological/metabolic adjustments allowing cellular stress adaptation and gene expression changes being used by organisms to achieve such adjustments

may significantly impact our ability to understand and/or guide evolution. Here, we studied those relationships during adaptation to various stress challenges in Saccharomyces cerevisiae, focusing on heat stress responses. We combined dozens of independent experiments measuring whole-genome gene expression changes during stress responses with a simplified kinetic model of central metabolism. We identified alternative quantitative ranges for a set of physiological variables in the model (production of ATP, trehalose, NADH, etc.) that are specific for adaptation to either heat stress or desiccation/rehydration. Our approach is scalable to other adaptive responses and could assist in developing biotechnological applications to manipulate cells for medical, biotechnological, or synthetic biology purposes.

URI

http://hdl.handle.net/10459.1/62832

DOI

https://doi.org/10.1016/j.celrep.2018.02.020

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Cell Reports, 2018, vol. 22, núm. 9, p. 2421-2430

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Except where otherwise noted, this item's license is described as cc-by-nc-nd (c) Pereira et al., 2018